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PXD000513

PXD000513 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhoto-cross-linking mass spectrometry for single amino acid assignment of RNA-binding sites in RNA-binding proteins
DescriptionMass spectrometry data of peptide–RNA cross-links derived from complex biological systems. Yeast (pre-)mRNPs were isolated by TAP tag purification of Cbp20; human protein–RNA complexes were assembled on an aptamer tagged pre-mRNA. Isolated complexes were UV irradiated. For each of the aforementioned experiments, a non-irradiated control was prepared. Yeast RBPs cross-linked in vivo after labeling with 4-thio-uridine (4SU) were isolated with oligo d(T). All samples were enriched for cross-linked heteroconjugates prior to LC-MS analysis.
HostingRepositoryPRIDE
AnnounceDate2014-10-23
AnnouncementXMLSubmission_2014-10-23_02:44:28.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTimo Sachsenberg
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-11 07:31:57ID requested
12014-08-07 01:12:09announced
22014-08-07 06:52:34announcedUpdated project metadata.
32014-10-23 02:44:29announcedUpdated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: human, RNP, cross-linking, mass spectrometry
Contact List
Oliver Kohlbacher
contact affiliationUniversity of Tübingen
contact emailoliver.kohlbacher@uni-tuebingen.de
lab head
Timo Sachsenberg
contact affiliationUniversity of Tübingen
contact emailsachsenb@informatik.uni-tuebingen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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