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PXD000498-3

PXD000498 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe quantitative and condition-dependent Escherichia coli proteome
DescriptionMeasuring precise concentrations of proteins can provide insights into biological processes. Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art quantitative mass spectrometry techniques to generate a comprehensive, condition-dependent protein-abundance map for Escherichia coli. We measure cellular protein concentrations for 55% of predicted E. coli genes (>2,300 proteins) under 22 different experimental conditions and identify methylation and N-terminal protein acetylations previously not known to be prevalent in bacteria. We uncover system-wide proteome allocation, expression regulation and post-translational adaptations. These data provide a valuable resource for the systems biology and broader E. coli research communities.
HostingRepositoryPRIDE
AnnounceDate2015-12-03
AnnouncementXMLSubmission_2015-12-08_08:13:34.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000498
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Schmidt
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-10-07 03:35:45ID requested
12015-10-12 04:34:39announced
22015-12-03 00:30:38announcedUpdated project metadata.
32015-12-08 08:13:35announcedUpdated project metadata.
Publication List
Schmidt A, Kochanowski K, Vedelaar S, Ahrn, é E, Volkmer B, Callipo L, Knoops K, Bauer M, Aebersold R, Heinemann M, The quantitative and condition-dependent Escherichia coli proteome. Nat Biotechnol, 34(1):104-10(2016) [pubmed]
Keyword List
curator keyword: Biological, Technical
submitter keyword: Absolute quantiifcation, iBAQ, mass spectreomtry, E. coli
Contact List
Alexander Schmidt
contact affiliationBiozentrum, University of Basel, 4056 Basel, Switzerland
contact emailalex.schmidt@unibas.ch
lab head
Alexander Schmidt
contact affiliationProteomics Core Facility
contact emailalex.schmidt@unibas.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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