PXD000493-3

PXD000493 is an original dataset announced via ProteomeXchange.

Title	Proteomics dataset for interphase chromatin probabilities
Description	Chromatin proteins mediate replication, regulate expression and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle it defies classical organellar proteomics and cannot be described by a single and ultimate list of protein components. Instead we propose a new approach that provides a quantitative assessment of a protein’s probability to function in chromatin. We integrate chromatin composition over a range of different biochemical and biological conditions. This resulted in interphase chromatin probabilities for 7635 human proteins, including 1840 previously uncharacterized proteins. Quantitative protein ontologies may provide a general alternative to list-based investigations of organelles and complement Gene Ontology.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-25_01:40:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georg Kustatscher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-10-03 08:24:37	ID requested
1	2014-02-18 07:49:57	announced
2	2014-04-10 05:53:30	announced	Updated publication reference for PubMed record(s): 24534090.
⏵ 3	2014-07-25 01:40:24	announced	Updated project metadata.

Kustatscher G, H, é, garat N, Wills KL, Furlan C, Bukowski-Wills JC, Hochegger H, Rappsilber J, Proteomics of a fuzzy organelle: interphase chromatin. EMBO J, 33(6):648-64(2014) [pubmed]

submitter keyword: Human, interphase, chromatin, ICPs

Juri Rappsilber
contact affiliation	Wellcome Trust Centre for Cell Biology
contact email	juri.rappsilber@ed.ac.uk
lab head
Georg Kustatscher
contact affiliation	Wellcome Trust Centre for Cell Biology
contact email	georg.kustatscher@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/02/PXD000493

PRIDE project URI

• PRIDE
  1. PXD000493
     1. Label: PRIDE project
     2. Name: Proteomics dataset for interphase chromatin probabilities