PXD000493 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics dataset for interphase chromatin probabilities |
Description | Chromatin proteins mediate replication, regulate expression and ensure integrity of the genome. So far, a comprehensive inventory of interphase chromatin has not been determined. This is largely due to its heterogeneous and dynamic composition, which makes conclusive biochemical purification difficult, if not impossible. As a fuzzy organelle it defies classical organellar proteomics and cannot be described by a single and ultimate list of protein components. Instead we propose a new approach that provides a quantitative assessment of a protein’s probability to function in chromatin. We integrate chromatin composition over a range of different biochemical and biological conditions. This resulted in interphase chromatin probabilities for 7635 human proteins, including 1840 previously uncharacterized proteins. Quantitative protein ontologies may provide a general alternative to list-based investigations of organelles and complement Gene Ontology. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-25 |
AnnouncementXML | Submission_2014-07-25_01:40:23.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Georg Kustatscher |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-10-03 08:24:37 | ID requested | |
1 | 2014-02-18 07:49:57 | announced | |
2 | 2014-04-10 05:53:30 | announced | Updated publication reference for PubMed record(s): 24534090. |
⏵ 3 | 2014-07-25 01:40:24 | announced | Updated project metadata. |
Publication List
Kustatscher G, H, é, garat N, Wills KL, Furlan C, Bukowski-Wills JC, Hochegger H, Rappsilber J, Proteomics of a fuzzy organelle: interphase chromatin. EMBO J, 33(6):648-64(2014) [pubmed] |
Keyword List
submitter keyword: Human, interphase, chromatin, ICPs |
Contact List
Juri Rappsilber |
contact affiliation | Wellcome Trust Centre for Cell Biology |
contact email | juri.rappsilber@ed.ac.uk |
lab head | |
Georg Kustatscher |
contact affiliation | Wellcome Trust Centre for Cell Biology |
contact email | georg.kustatscher@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000493
- Label: PRIDE project
- Name: Proteomics dataset for interphase chromatin probabilities