PXD000479-3
PXD000479 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Annotation of the zebrafish genome through an integrated transcriptomic and proteomic analysis |
Description | Zebrafish organ proteomics was carried out for proteogenomic analysis. High resolution mass spectrometry-based proteomic profiling of 11 adult organs (eye, brain, liver, spleen, intestine-pancreas, ovary, testes, muscle, heart and head) and two developmental stages (embryos 48 and 120 hours post-fertilization) of zebrafish (SAT line) was carried out. Protein extracts were reduced and alkylated (iodoacetamide). Off-loine fractionation was carried out by SDS-PAGE and basic RPLC. MS analysis was cariied on Agilent 6540 Q-TOF. Spleen and Testis samples were also analyzed on Thermo Orbitrap LTQ Velos. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-25 |
AnnouncementXML | Submission_2014-07-28_02:23:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Akhilesh Pandey |
SpeciesList | scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; |
ModificationList | iodoacetamide derivatized residue |
Instrument | 6520 Quadrupole Time-of-Flight LC/MS; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2013-09-23 02:54:37 | ID requested | |
1 | 2014-02-26 07:58:43 | announced | |
2 | 2014-07-25 01:39:07 | announced | Updated project metadata. |
⏵ 3 | 2014-07-28 02:23:03 | announced | Updated project metadata. |
Publication List
Kelkar DS, Provost E, Chaerkady R, Muthusamy B, Manda SS, Subbannayya T, Selvan LD, Wang CH, Datta KK, Woo S, Dwivedi SB, Renuse S, Getnet D, Huang TC, Kim MS, Pinto SM, Mitchell CJ, Madugundu AK, Kumar P, Sharma J, Advani J, Dey G, Balakrishnan L, Syed N, Nanjappa V, Subbannayya Y, Goel R, Prasad TS, Bafna V, Sirdeshmukh R, Gowda H, Wang C, Leach SD, Pandey A, Annotation of the zebrafish genome through an integrated transcriptomic and proteomic analysis. Mol Cell Proteomics, 13(11):3184-98(2014) [pubmed] |
Keyword List
submitter keyword: zebrafish, Danio rerio, proteogenomics, embryo, Liver, Kidney, spleen, intestine-pancreas, whole body, brain, eye, muscle, ovary, testis |
Contact List
Akhilesh Pandey | |
---|---|
contact affiliation | Institute of Genetic Medicine |
contact email | pandey@jhmi.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/02/PXD000479 |
PRIDE project URI |
Repository Record List
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