PXD000479

PXD000479 is an original dataset announced via ProteomeXchange.

Title	Annotation of the zebrafish genome through an integrated transcriptomic and proteomic analysis
Description	Zebrafish organ proteomics was carried out for proteogenomic analysis. High resolution mass spectrometry-based proteomic profiling of 11 adult organs (eye, brain, liver, spleen, intestine-pancreas, ovary, testes, muscle, heart and head) and two developmental stages (embryos 48 and 120 hours post-fertilization) of zebrafish (SAT line) was carried out. Protein extracts were reduced and alkylated (iodoacetamide). Off-loine fractionation was carried out by SDS-PAGE and basic RPLC. MS analysis was cariied on Agilent 6540 Q-TOF. Spleen and Testis samples were also analyzed on Thermo Orbitrap LTQ Velos.
HostingRepository	PRIDE
AnnounceDate	2014-07-25
AnnouncementXML	Submission_2014-07-28_02:23:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	iodoacetamide derivatized residue
Instrument	6520 Quadrupole Time-of-Flight LC/MS; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-23 02:54:37	ID requested
1	2014-02-26 07:58:43	announced
2	2014-07-25 01:39:07	announced	Updated project metadata.
⏵ 3	2014-07-28 02:23:03	announced	Updated project metadata.

Kelkar DS, Provost E, Chaerkady R, Muthusamy B, Manda SS, Subbannayya T, Selvan LD, Wang CH, Datta KK, Woo S, Dwivedi SB, Renuse S, Getnet D, Huang TC, Kim MS, Pinto SM, Mitchell CJ, Madugundu AK, Kumar P, Sharma J, Advani J, Dey G, Balakrishnan L, Syed N, Nanjappa V, Subbannayya Y, Goel R, Prasad TS, Bafna V, Sirdeshmukh R, Gowda H, Wang C, Leach SD, Pandey A, Annotation of the zebrafish genome through an integrated transcriptomic and proteomic analysis. Mol Cell Proteomics, 13(11):3184-98(2014) [pubmed]

submitter keyword: zebrafish, Danio rerio, proteogenomics, embryo, Liver, Kidney, spleen, intestine-pancreas, whole body, brain, eye, muscle, ovary, testis

Akhilesh Pandey
contact affiliation	Institute of Genetic Medicine
contact email	pandey@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/02/PXD000479

PRIDE project URI

• PRIDE
  1. PXD000479
     1. Label: PRIDE project
     2. Name: Annotation of the zebrafish genome through an integrated transcriptomic and proteomic analysis