PXD000464-1

PXD000464 is an original dataset announced via ProteomeXchange.

Title	LC-MS data of peptides from p53 isolated by custom immunoaffinity chromatography
Description	We exploited a primary human fibroblast system in which the p53 tumor suppressor protein accumulates to high concentrations due to infection by an adenovirus type 5 mutant that lacks the E1B 55kDa E3 ubiquitin ligase, which targets p53 for degradation, to examine select post-translational modifications (PTMs) present on this transcriptionally inactivated endogenous human p53, as well as on p53 activated in response to etoposide treatment. These forms of p53 were isolated from whole cell lysates by immunoaffinity chromatography using a cocktail of monoclonal antibodies, followed by SDS-PAGE, and peptides derived from these species were subjected to nano-ultra-high performance liquid chromatography-MS and MS/MS analyses on high resolution Orbitrap platforms. Proteomics informatics: Raw LC-MS/MS data were processed into mgf-format peaklist files and searched using Mascot (v. 2.2.7) against the human p53 sequence determined by direct sequencing of the p53 gene from cDNA derived from the specific batch of HFFs used in this study (identical to the consensus sequence for UniProt P04637), employing a window of 8 ppm for the precursor and 1.2 Da for the fragment ion species and allowing for <= 3 missed trypsin and semi-trypsin cleavages, carbamidomethylation of cysteines as a fixed modification, with methionine oxidation, N-terminal protein acetylation, phosphorylation of Ser and Thr, acetylation of Lys, methylation, dimethylation, and trimethylation of Lys and Arg, diglycine modification of Lys (ubiquitinylation), and deamidation of Asn and Gln, iteratively as variable modifications. Aggregate search results for each sample were collated and consolidated using Scaffold (vs. 4.0.7) according to the PeptideProphet and ProteinProphet parsimony algorithms implemented therein, and filtered to the 90% peptide confidence level and to a precursor mass accuracy within 2-3 ppm.
HostingRepository	PRIDE
AnnounceDate	2013-10-17
AnnouncementXML	Submission_2013-10-17_05:51:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000464
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Perlman
SpeciesList	scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534;
ModificationList	iodoacetamide derivatized residue; dehydrated residue; monomethylated residue; fragment neutral loss: 63.998283; monohydroxylated residue; deaminated residue; phosphorylated residue; fragment neutral loss: 97.9769; acetylated residue; formylated residue; ubiquitination signature dipeptidyl lysine
Instrument	radial ejection linear ion trap

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-13 07:55:23	ID requested
⏵ 1	2013-10-17 05:51:50	announced
⏵ 1	2013-10-17 06:17:51	announced
2	2014-07-28 00:48:23	announced	Updated project metadata.

Dataset with its publication pending

submitter keyword: p53 Post-translational Modification

Dr. David H. Perlman
contact attribute	perlmand@princeton.edu
contact affiliation	Princeton University
contact email	perlmand@princeton.edu
David Perlman
contact affiliation	Princeton University Molecular Biology
contact email	PerlmanD@Princeton.edu

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/10/PXD000464

• PRIDE
  1. 31278
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Ep53 3-1 pt1
     3. ModificationList: fragment neutral loss, monohydroxylated residue, iodoacetamide derivatized residue, ubiquitination signature dipeptidyl lysine, formylated residue, phosphorylated residue, fragment neutral loss, acetylated residue, monomethylated residue, dehydrated residue, deaminated residue,
     4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
     5. PublicationRef: pending
  2. 31292
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Delta E1B p53 3-4 pt2
     3. ModificationList: monomethylated residue, phosphorylated residue, fragment neutral loss, iodoacetamide derivatized residue,
     4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
     5. PublicationRef: pending
  3. 31299
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Delta E1B p53 1 Tech Rep A pt2
     3. ModificationList: iodoacetamide derivatized residue, phosphorylated residue, fragment neutral loss, acetylated residue, monomethylated residue, ubiquitination signature dipeptidyl lysine, formylated residue, deaminated residue, fragment neutral loss, monohydroxylated residue, dehydrated residue,
     4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
     5. PublicationRef: pending
  4. 31297
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Delta E1B p53 3-7 pt1
     3. ModificationList: ubiquitination signature dipeptidyl lysine, iodoacetamide derivatized residue, acetylated residue, phosphorylated residue, fragment neutral loss, monomethylated residue, formylated residue, fragment neutral loss, monohydroxylated residue, dehydrated residue, deaminated residue,
     4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
     5. PublicationRef: pending
  5. 31285
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Delta E1B p53 2-3 pt1
     3. ModificationList: iodoacetamide derivatized residue, ubiquitination signature dipeptidyl lysine, formylated residue, acetylated residue, phosphorylated residue, fragment neutral loss, fragment neutral loss, monohydroxylated residue, monomethylated residue, deaminated residue,
     4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
     5. PublicationRef: pending
  6. 31263
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Delta E1B p53 3-1 pt2
     3. ModificationList: fragment neutral loss, monohydroxylated residue, iodoacetamide derivatized residue, monomethylated residue, acetylated residue, dehydrated residue, phosphorylated residue, fragment neutral loss, formylated residue, deaminated residue, ubiquitination signature dipeptidyl lysine,
     4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
     5. PublicationRef: pending
  7. 31276
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Delta E1B p53 3-2 pt1
     3. ModificationList: monomethylated residue, phosphorylated residue, fragment neutral loss, acetylated residue, formylated residue, fragment neutral loss, monohydroxylated residue, iodoacetamide derivatized residue, ubiquitination signature dipeptidyl lysine, deaminated residue, dehydrated residue,
     4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
     5. PublicationRef: pending
  8. 31295
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Delta E1B p53 3-6 pt1
     3. ModificationList: acetylated residue, ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, deaminated residue, fragment neutral loss, monohydroxylated residue, monomethylated residue, iodoacetamide derivatized residue, dehydrated residue, formylated residue,
     4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
     5. PublicationRef: pending
  9. 31281
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Delta E1B p53 2-1 pt1
     3. ModificationList: ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, fragment neutral loss, monohydroxylated residue,
     4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
     5. PublicationRef: pending
  10. 31275
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 3-2 pt2
      3. ModificationList: monomethylated residue, phosphorylated residue, fragment neutral loss, acetylated residue, formylated residue, fragment neutral loss, monohydroxylated residue, ubiquitination signature dipeptidyl lysine, deaminated residue, iodoacetamide derivatized residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  11. 31296
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 3-6 pt2
      3. ModificationList: acetylated residue, ubiquitination signature dipeptidyl lysine, fragment neutral loss, monohydroxylated residue, phosphorylated residue, fragment neutral loss, monomethylated residue, iodoacetamide derivatized residue, formylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  12. 31273
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 2-2 pt2
      3. ModificationList: ubiquitination signature dipeptidyl lysine, formylated residue, monomethylated residue, phosphorylated residue, fragment neutral loss, acetylated residue, iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  13. 31260
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Cos-1 p53 Tech Rep B pt1
      3. ModificationList: acetylated residue, fragment neutral loss, monohydroxylated residue, iodoacetamide derivatized residue, ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, formylated residue, monomethylated residue, dehydrated residue, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  14. 31267
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 1 Tech Rep B pt2
      3. ModificationList: monomethylated residue, ubiquitination signature dipeptidyl lysine, formylated residue, phosphorylated residue, fragment neutral loss, acetylated residue, iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, dehydrated residue, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  15. 31264
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 3-1 pt1
      3. ModificationList: fragment neutral loss, monohydroxylated residue, iodoacetamide derivatized residue, deaminated residue, dehydrated residue, monomethylated residue, phosphorylated residue, fragment neutral loss, ubiquitination signature dipeptidyl lysine, acetylated residue, formylated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  16. 31259
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Cos-1 p53 Tech Rep B pt2
      3. ModificationList: iodoacetamide derivatized residue, phosphorylated residue, fragment neutral loss, monomethylated residue, dehydrated residue, fragment neutral loss, monohydroxylated residue, deaminated residue, acetylated residue, formylated residue, ubiquitination signature dipeptidyl lysine,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  17. 31288
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 2-4 pt2
      3. ModificationList: iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, ubiquitination signature dipeptidyl lysine, formylated residue, phosphorylated residue, fragment neutral loss, monomethylated residue, deaminated residue, acetylated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  18. 31261
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Cos-1 p53 Tech Rep C pt2
      3. ModificationList: iodoacetamide derivatized residue, phosphorylated residue, fragment neutral loss, monomethylated residue, dehydrated residue, deaminated residue, fragment neutral loss, monohydroxylated residue, acetylated residue, formylated residue, ubiquitination signature dipeptidyl lysine,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  19. 31274
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 2-2 pt1
      3. ModificationList: ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, acetylated residue, formylated residue, monomethylated residue, iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  20. 31269
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 1 Tech Rep C pt2
      3. ModificationList: monomethylated residue, acetylated residue, iodoacetamide derivatized residue, formylated residue, fragment neutral loss, monohydroxylated residue, phosphorylated residue, fragment neutral loss, ubiquitination signature dipeptidyl lysine, dehydrated residue, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  21. 31300
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 1 Tech Rep A pt1
      3. ModificationList: iodoacetamide derivatized residue, acetylated residue, ubiquitination signature dipeptidyl lysine, formylated residue, phosphorylated residue, fragment neutral loss, monomethylated residue, deaminated residue, dehydrated residue, fragment neutral loss, monohydroxylated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  22. 31291
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 3-4 pt1
      3. ModificationList: phosphorylated residue, fragment neutral loss, monomethylated residue, formylated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  23. 31294
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 3-5 pt2
      3. ModificationList: monomethylated residue, phosphorylated residue, fragment neutral loss, formylated residue, iodoacetamide derivatized residue, acetylated residue, fragment neutral loss, monohydroxylated residue, ubiquitination signature dipeptidyl lysine, dehydrated residue, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  24. 31282
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 2-1 pt2
      3. ModificationList: ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, fragment neutral loss, monohydroxylated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  25. 31279
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 3-2 pt2
      3. ModificationList: monomethylated residue, phosphorylated residue, fragment neutral loss, formylated residue, iodoacetamide derivatized residue, acetylated residue, ubiquitination signature dipeptidyl lysine, deaminated residue, fragment neutral loss, monohydroxylated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  26. 31298
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 3-7 pt2
      3. ModificationList: ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, monomethylated residue, iodoacetamide derivatized residue, acetylated residue, formylated residue, fragment neutral loss, monohydroxylated residue, dehydrated residue, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  27. 31268
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 1 Tech Rep B pt1
      3. ModificationList: monomethylated residue, ubiquitination signature dipeptidyl lysine, formylated residue, phosphorylated residue, fragment neutral loss, iodoacetamide derivatized residue, acetylated residue, fragment neutral loss, monohydroxylated residue, dehydrated residue, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  28. 31301
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 1 Tech Rep B pt2
      3. ModificationList: acetylated residue, iodoacetamide derivatized residue, formylated residue, fragment neutral loss, monohydroxylated residue, monomethylated residue, phosphorylated residue, fragment neutral loss, deaminated residue, ubiquitination signature dipeptidyl lysine, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  29. 31271
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 2-1 pt2
      3. ModificationList: formylated residue, monomethylated residue, ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, iodoacetamide derivatized residue, acetylated residue, fragment neutral loss, monohydroxylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  30. 31284
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 2-2 pt2
      3. ModificationList: iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, formylated residue, monomethylated residue, acetylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  31. 31293
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 3-5 pt1
      3. ModificationList: ubiquitination signature dipeptidyl lysine, acetylated residue, formylated residue, phosphorylated residue, fragment neutral loss, monomethylated residue, iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  32. 31290
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 3-3 pt2
      3. ModificationList: formylated residue, phosphorylated residue, fragment neutral loss, acetylated residue, monomethylated residue, ubiquitination signature dipeptidyl lysine, iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  33. 31304
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 1 Tech Rep C pt1
      3. ModificationList: iodoacetamide derivatized residue, formylated residue, fragment neutral loss, monohydroxylated residue, phosphorylated residue, fragment neutral loss, monomethylated residue, acetylated residue, deaminated residue, ubiquitination signature dipeptidyl lysine, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  34. 31303
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 1 Tech Rep C pt2
      3. ModificationList: iodoacetamide derivatized residue, phosphorylated residue, fragment neutral loss, monomethylated residue, formylated residue, acetylated residue, deaminated residue, dehydrated residue, ubiquitination signature dipeptidyl lysine, fragment neutral loss, monohydroxylated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  35. 31257
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Cos-1 p53 Tech Rep A pt2
      3. ModificationList: iodoacetamide derivatized residue, dehydrated residue, monomethylated residue, fragment neutral loss, monohydroxylated residue, deaminated residue, phosphorylated residue, fragment neutral loss, acetylated residue, formylated residue, ubiquitination signature dipeptidyl lysine,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  36. 31302
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 1 Tech Rep B pt1
      3. ModificationList: iodoacetamide derivatized residue, formylated residue, fragment neutral loss, monohydroxylated residue, monomethylated residue, phosphorylated residue, fragment neutral loss, acetylated residue, ubiquitination signature dipeptidyl lysine, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  37. 31266
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 1 Tech Rep A pt1
      3. ModificationList: fragment neutral loss, monohydroxylated residue, phosphorylated residue, fragment neutral loss, ubiquitination signature dipeptidyl lysine, formylated residue, iodoacetamide derivatized residue, monomethylated residue, acetylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  38. 31286
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 2-3 pt2
      3. ModificationList: iodoacetamide derivatized residue, ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, monomethylated residue, acetylated residue, formylated residue, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  39. 31283
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 2-2 pt1
      3. ModificationList: iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, ubiquitination signature dipeptidyl lysine, formylated residue, acetylated residue, phosphorylated residue, fragment neutral loss, monomethylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  40. 31277
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 3-1 pt2
      3. ModificationList: fragment neutral loss, monohydroxylated residue, iodoacetamide derivatized residue, ubiquitination signature dipeptidyl lysine, formylated residue, acetylated residue, monomethylated residue, phosphorylated residue, fragment neutral loss, dehydrated residue, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  41. 31262
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Cos-1 p53 Tech Rep C pt1
      3. ModificationList: acetylated residue, fragment neutral loss, monohydroxylated residue, monomethylated residue, iodoacetamide derivatized residue, phosphorylated residue, fragment neutral loss, formylated residue, ubiquitination signature dipeptidyl lysine, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  42. 31258
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Cos-1 p53 Tech Rep A pt1
      3. ModificationList: acetylated residue, fragment neutral loss, monohydroxylated residue, iodoacetamide derivatized residue, phosphorylated residue, fragment neutral loss, monomethylated residue, formylated residue, dehydrated residue, ubiquitination signature dipeptidyl lysine, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  43. 31272
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 2-1 pt1
      3. ModificationList: formylated residue, monomethylated residue, phosphorylated residue, fragment neutral loss, acetylated residue, ubiquitination signature dipeptidyl lysine, iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  44. 31287
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 2-4 pt1
      3. ModificationList: iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, ubiquitination signature dipeptidyl lysine, phosphorylated residue, fragment neutral loss, formylated residue, monomethylated residue, acetylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  45. 31265
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 1 Tech Rep A pt2
      3. ModificationList: fragment neutral loss, monohydroxylated residue, phosphorylated residue, fragment neutral loss, ubiquitination signature dipeptidyl lysine, acetylated residue, formylated residue, iodoacetamide derivatized residue, monomethylated residue, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  46. 31280
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 3-2 pt1
      3. ModificationList: acetylated residue, monomethylated residue, phosphorylated residue, fragment neutral loss, formylated residue, iodoacetamide derivatized residue, fragment neutral loss, monohydroxylated residue, ubiquitination signature dipeptidyl lysine, deaminated residue, dehydrated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  47. 31289
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Delta E1B p53 3-3 pt1
      3. ModificationList: formylated residue, phosphorylated residue, fragment neutral loss, acetylated residue, monomethylated residue, iodoacetamide derivatized residue, ubiquitination signature dipeptidyl lysine, fragment neutral loss, monohydroxylated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending
  48. 31270
      1. InstrumentRef: INSTRUMENT_1
      2. Label: Ep53 1 Tech Rep C pt1
      3. ModificationList: monomethylated residue, iodoacetamide derivatized residue, formylated residue, acetylated residue, fragment neutral loss, monohydroxylated residue, phosphorylated residue, fragment neutral loss, ubiquitination signature dipeptidyl lysine, dehydrated residue, deaminated residue,
      4. Name: Tandem mass spectral assignments for peptides derived from p53 protein isolates subjected to nano-UPLC-Orbitrap-MS analysis
      5. PublicationRef: pending