PXD000441 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human AML cell lines LC-MS/MS |
Description | As a direct consequence of the high diversity of the aggressive blood cancer acute myeloid leukemia (AML), proteomic samples from patients are strongly heterogeneous, rendering their accurate relative quantification challenging. In the present study, we investigated the benefits of using a super-SILAC mix of AML derived cell lines as internal standard for quantitative shotgun studies. The Molm-13, NB4, MV4-11, THP-1, and OCI-AML3 cell lines were selected for their complementarity with regard to clinical, cytogenetic and molecular risk factors used for prognostication of AML patients. The resulting internal standard presents a high coverage of the AML proteome compared to single cell lines allied with high technical reproducibility, thus enabling its use for AML patient comparison. This was confirmed by comparing the protein regulation between the five cell lines and applying the internal standard to patient material. |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-15 |
AnnouncementXML | Submission_2014-07-15_05:36:24.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Elise Aasebø |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; 6x(13)C labeled L-arginine; 6x(13)C: 2x(15)N labeled L-lysine |
Instrument | LTQ Orbitrap Velos; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-09-02 07:18:03 | ID requested | |
⏵ 1 | 2014-07-15 05:36:25 | announced | |
2 | 2014-07-24 05:20:19 | announced | Updated project metadata. |
3 | 2014-08-05 03:34:49 | announced | Updated project metadata. |
4 | 2014-10-23 02:44:34 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, AML, cell lines, super-SILAC, LC-MS, Orbitrap velos pro |
Contact List
Frode Steingrimsen Berven |
contact affiliation | Department of Biomedicine, PROBE, University of Bergen, Norway |
contact email | frode.berven@biomed.uib.no |
lab head | |
Elise Aasebø |
contact affiliation | PROBE |
contact email | elise.aasebo@biomed.uib.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000441
- Label: PRIDE project
- Name: Human AML cell lines LC-MS/MS