PXD000441

PXD000441 is an original dataset announced via ProteomeXchange.

Title	Human AML cell lines LC-MS/MS
Description	As a direct consequence of the high diversity of the aggressive blood cancer acute myeloid leukemia (AML), proteomic samples from patients are strongly heterogeneous, rendering their accurate relative quantification challenging. In the present study, we investigated the benefits of using a super-SILAC mix of AML derived cell lines as internal standard for quantitative shotgun studies. The Molm-13, NB4, MV4-11, THP-1, and OCI-AML3 cell lines were selected for their complementarity with regard to clinical, cytogenetic and molecular risk factors used for prognostication of AML patients. The resulting internal standard presents a high coverage of the AML proteome compared to single cell lines allied with high technical reproducibility, thus enabling its use for AML patient comparison. This was confirmed by comparing the protein regulation between the five cell lines and applying the internal standard to patient material.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_02:44:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elise Aasebø
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; 6x(13)C labeled L-arginine; 6x(13)C: 2x(15)N labeled L-lysine
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-02 07:18:03	ID requested
1	2014-07-15 05:36:25	announced
2	2014-07-24 05:20:19	announced	Updated project metadata.
3	2014-08-05 03:34:49	announced	Updated project metadata.
⏵ 4	2014-10-23 02:44:34	announced	Updated project metadata.

Aaseb, ø E, Vaudel M, Mjaavatten O, Gausdal G, Van der Burgh A, Gjertsen BT, D, ø, skeland SO, Bruserud O, Berven FS, Selheim F, Performance of super-SILAC based quantitative proteomics for comparison of different acute myeloid leukemia (AML) cell lines. Proteomics, 14(17-18):1971-6(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, AML, cell lines, super-SILAC, LC-MS, Orbitrap velos pro

Frode Steingrimsen Berven
contact affiliation	Department of Biomedicine, PROBE, University of Bergen, Norway
contact email	frode.berven@biomed.uib.no
lab head
Elise Aasebø
contact affiliation	PROBE
contact email	elise.aasebo@biomed.uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/07/PXD000441

PRIDE project URI

• PRIDE
  1. PXD000441
     1. Label: PRIDE project
     2. Name: Human AML cell lines LC-MS/MS