PXD000440-1

PXD000440 is an original dataset announced via ProteomeXchange.

Title	Qualitatively Different T Cell Phenotypic Responses to IL-2 versus IL-15 are Unified by Identical Dependences on Receptor Signal Strength and Duration
Description	3 biological replicates of F15R-Kit cells stimulated with 500pM IL-2 or IL-15 for 15 minutes, and two biological replicates of unstimulated cells (total 8 conditions) were iTRAQ8plex labeled, combined and analyzed together. Phosphotyrosine peptides were enriched for by immunoprecipitation followed by further enrichment by IMAC. Peptides were anaylzed on an Orbitrap Elite with CID and HCD scans for MS/MS peptide identification.
HostingRepository	PRIDE
AnnounceDate	2013-11-07
AnnouncementXML	Submission_2013-11-07_04:17:44.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000440
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hannah Johnson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-cysteine glutathione disulfide; iodoacetamide derivatized residue; phosphorylated residue; neutral loss: 97.976896; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; neutral loss: 63.998285
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-02 02:04:35	ID requested
⏵ 1	2013-11-07 04:17:46	announced
2	2014-07-28 00:46:15	announced	Updated project metadata.
3	2014-08-08 05:44:45	announced	Updated project metadata.

Dataset with its publication pending

submitter keyword: iTRAQ8plex, Phosphotyrosine, Phosphorylation, Quantification, IL-2, IL-15,

Abhinav Arneja
contact attribute	abhinav@mit.edu
contact affiliation	MIT
contact email	abhinav@mit.edu
Forest M White
contact attribute	fwhite@MIT.EDU
contact affiliation	MIT
contact email	fwhite@MIT.EDU
Hannah Johnson
contact affiliation	Biological Engineering
contact email	hjohnsn@mit.edu

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• PRIDE
  1. 31010
     1. InstrumentRef: INSTRUMENT_1
     2. Label: iTRAQ8plex F15R-Kit cells IL-2 vs IL-15; Phosphotyrosine
     3. ModificationList: L-cysteine glutathione disulfide, iodoacetamide derivatized residue, phosphorylated residue, neutral loss, iTRAQ8plex-116 reporter+balance reagent acylated residue, monohydroxylated residue, neutral loss,
     4. Name: iTRAQ8plex labelled F15R-Kit cells stimulated with control (n=2), IL-2 (n=3) or IL15 (n=3); Phosphotyrosine analysis
     5. PublicationRef: pending