PXD000440

PXD000440 is an original dataset announced via ProteomeXchange.

Title	Qualitatively Different T Cell Phenotypic Responses to IL-2 versus IL-15 are Unified by Identical Dependences on Receptor Signal Strength and Duration
Description	3 biological replicates of F15R-Kit cells stimulated with 500pM IL-2 or IL-15 for 15 minutes, and two biological replicates of unstimulated cells (total 8 conditions) were iTRAQ8plex labeled, combined and analyzed together. Phosphotyrosine peptides were enriched for by immunoprecipitation followed by further enrichment by IMAC. Peptides were anaylzed on an Orbitrap Elite with CID and HCD scans for MS/MS peptide identification.
HostingRepository	PRIDE
AnnounceDate	2014-07-28
AnnouncementXML	Submission_2014-08-08_05:44:44.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000440
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hannah Johnson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; phosphorylated residue; iodoacetamide derivatized residue; L-cysteine glutathione disulfide
Instrument	instrument model: Orbitrap Elite CID/HCD

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-02 02:04:35	ID requested
1	2013-11-07 04:17:46	announced
2	2014-07-28 00:46:15	announced	Updated project metadata.
⏵ 3	2014-08-08 05:44:45	announced	Updated project metadata.

Arneja A, Johnson H, Gabrovsek L, Lauffenburger DA, White FM, Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration. J Immunol, 192(1):123-35(2014) [pubmed]

submitter keyword: iTRAQ8plex, Phosphotyrosine, Phosphorylation, Quantification, IL-2, IL-15,

Hannah Johnson
contact affiliation	Biological Engineering
contact email	hjohnsn@mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/11/PXD000440

PRIDE project URI

• PRIDE
  1. PXD000440
     1. Label: PRIDE project
     2. Name: Qualitatively Different T Cell Phenotypic Responses to IL-2 versus IL-15 are Unified by Identical Dependences on Receptor Signal Strength and Duration