PXD000399-1

PXD000399 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative LC-MS/MS Analysis of Apical Membrane Proteome of mpkCCD Cells
Description	This is a 2-plex SILAC-based quantitative proteomic study. It quantifies abundance changes of the apical membrane proteins of the kidney collecting duct model cells (mpkCCD) in response to vasopressin. Cells were labeled with normal or heavy lysine and arginine and then exposed to vehicle or the vasopressin analogy dDAVP (1 nM, 1 hour). Apical membrane proteins of vehicle and dDAVP-treated cells were enriched via surface biotinylation and affinity purification prior to LC-MS/MS analysis. Database search and protein quantification were performed using the SEQUEST algorithm included in the Proteome Discover Version 1.3 (Thermo Scientific). The database used for the spectral searching was prepared from the mouse RefSeq database plus common contaminants.
HostingRepository	PRIDE
AnnounceDate	2013-10-02
AnnouncementXML	Submission_2013-10-03_00:15:27.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ming-Jiun Yu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2013-08-05 02:43:47	ID requested
⏵ 1	2013-10-03 00:15:33	announced
2	2014-05-19 07:07:57	announced	Updated publication reference for PubMed record(s): 24085853.
3	2014-07-25 01:30:06	announced	Updated project metadata.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: SILAC, collecting duct, vasopressin, aquaporin-2, actin dynamics

submitter keyword: SILAC, collecting duct, vasopressin, aquaporin-2, actin dynamics

Contact List

Ming-Jiun Yu
contact affiliation	Institute of Biochemistry and Molecular Biology, National Taiwan University College of Medicine
contact email	mjyu@ntu.edu.tw

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/10/PXD000399

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/10/PXD000399