PXD000378 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Functional analysis of the rodent tau mutation in a marine unicellular alga |
Description | Casein Kinase 1 (CK1) is one of few proteins known to affect cellular timekeeping across metazoans, and the naturally occurring CK1tau mutation shortens circadian period in mammals. Functional conservation of a timekeeping function for CK1 in the green lineage was recently identified in the green marine unicell Ostreococcus tauri, in spite of the absence of CK1's transcriptional targets known from other species. The short-period phenotype of CK1tau mutant in mammals depends specifically on increased CK1 activity against PERIOD proteins. To understand how CK1 acts differently upon the algal clock, we analysed the effect of CK1tau overexpression on the phospho-preoteome of O. tauri using label-free quantification compared to the parent line. Ostreococcus tauri cells were harvested, trypsinised and phosphopeptide enrichment was performed using TiO2 columns followed by LC-MS separation (140minute gradient) on a Orbitrap XL. All those steps are described previously (Le Bihan et al J Proteomics. 2011 Sep 6;74(10):2060-70). MSMS data was searched using MASCOT Version 2.4 (Matrix Science Ltd, UK) against the Ostreococcus tauri subset of the NCBI protein database (12/01/2011; 8,726 sequences) using a maximum missed-cut value of 2., variable oxidation (M), N-terminal protein acetylation, phosphorylation (STY) and fixed carbamidomethylation (C); precursor mass tolerance was 7 ppm and MSMS tolerance 0.4 amu. The significance threshold (p) was set below 0.05 (MudPIT scoring). |
HostingRepository | PRIDE |
AnnounceDate | 2014-07-28 |
AnnouncementXML | Submission_2014-07-28_00:43:00.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000378 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Thierry Le Bihan |
SpeciesList | scientific name: Ostreococcus tauri; NCBI TaxID: 70448; |
ModificationList | monohydroxylated residue; acetylated residue; phosphorylated residue; iodoacetamide derivatized residue |
Instrument | instrument model: OrbitrapXL; instrument model: IonTrap XCT Ultra |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-07-29 05:29:09 | ID requested | |
1 | 2013-10-18 02:36:04 | announced | |
1 | 2013-10-18 02:40:58 | announced | |
⏵ 2 | 2014-07-28 00:43:00 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Ostreococcus tauri, phosphopeptide enrichment, Tau mutation |
Contact List
Thierry Le Bihan |
contact affiliation | SynthSys: Synthetic & Systems Biology |
contact email | thierry.lebihan@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000378
- Label: PRIDE project
- Name: Functional analysis of the rodent tau mutation in a marine unicellular alga