PXD000378

PXD000378 is an original dataset announced via ProteomeXchange.

Title	Functional analysis of the rodent tau mutation in a marine unicellular alga
Description	Casein Kinase 1 (CK1) is one of few proteins known to affect cellular timekeeping across metazoans, and the naturally occurring CK1tau mutation shortens circadian period in mammals. Functional conservation of a timekeeping function for CK1 in the green lineage was recently identified in the green marine unicell Ostreococcus tauri, in spite of the absence of CK1's transcriptional targets known from other species. The short-period phenotype of CK1tau mutant in mammals depends specifically on increased CK1 activity against PERIOD proteins. To understand how CK1 acts differently upon the algal clock, we analysed the effect of CK1tau overexpression on the phospho-preoteome of O. tauri using label-free quantification compared to the parent line. Ostreococcus tauri cells were harvested, trypsinised and phosphopeptide enrichment was performed using TiO2 columns followed by LC-MS separation (140minute gradient) on a Orbitrap XL. All those steps are described previously (Le Bihan et al J Proteomics. 2011 Sep 6;74(10):2060-70). MSMS data was searched using MASCOT Version 2.4 (Matrix Science Ltd, UK) against the Ostreococcus tauri subset of the NCBI protein database (12/01/2011; 8,726 sequences) using a maximum missed-cut value of 2., variable oxidation (M), N-terminal protein acetylation, phosphorylation (STY) and fixed carbamidomethylation (C); precursor mass tolerance was 7 ppm and MSMS tolerance 0.4 amu. The significance threshold (p) was set below 0.05 (MudPIT scoring).
HostingRepository	PRIDE
AnnounceDate	2014-07-28
AnnouncementXML	Submission_2014-07-28_00:43:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000378
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thierry Le Bihan
SpeciesList	scientific name: Ostreococcus tauri; NCBI TaxID: 70448;
ModificationList	monohydroxylated residue; acetylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	instrument model: OrbitrapXL; instrument model: IonTrap XCT Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-29 05:29:09	ID requested
1	2013-10-18 02:36:04	announced
1	2013-10-18 02:40:58	announced
⏵ 2	2014-07-28 00:43:00	announced	Updated project metadata.

Dataset with its publication pending

submitter keyword: Ostreococcus tauri, phosphopeptide enrichment, Tau mutation

Thierry Le Bihan
contact affiliation	SynthSys: Synthetic & Systems Biology
contact email	thierry.lebihan@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/10/PXD000378

PRIDE project URI

• PRIDE
  1. PXD000378
     1. Label: PRIDE project
     2. Name: Functional analysis of the rodent tau mutation in a marine unicellular alga