PXD000355 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic identification of rainbow trout sperm proteins |
Description | Proteomics represents a powerful tool for the analysis of fish spermatozoa, since these cells are transcriptionally inactive. The aim of the present study was to generate an inventory of the most prominent rainbow trout sperm proteins with the use of one-dimensional electrophoresis prefractionation combined with performance liquid chromatography electrospray ionization tandem mass spectrometry. This study provides the first in-depth analysis of the rainbow trout sperm proteome, with a total of 204 identified proteins. We found that rainbow trout spermatozoa are equipped with functionally diverse proteins related to energetic metabolism, signal transduction, protein turnover, transport, cytoskeleton, oxidative injures and stress and reproduction. The availability of a catalogue of rainbow trout sperm proteins provides a crucial tool for the understanding of fundamental molecular processes in fish spermatozoa for ongoing research in the development of novel markers of sperm quality and for the optimization of short- and long-term sperm preservation procedures. |
HostingRepository | PRIDE |
AnnounceDate | 2013-11-05 |
AnnouncementXML | Submission_2013-11-05_01:35:37.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD000355 |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Joanna Nynca |
SpeciesList | scientific name: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri); NCBI TaxID: 8022; |
ModificationList | iodoacetamide derivatized residue; monohydroxylated residue; neutral loss: 63.998285 |
Instrument | radial ejection linear ion trap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-07-22 04:08:39 | ID requested | |
⏵ 1 | 2013-11-05 01:35:39 | announced | |
⏵ 1 | 2013-11-06 07:15:43 | announced | |
2 | 2014-04-02 06:56:11 | announced | Updated publication reference for PubMed record(s): 24687995. |
3 | 2014-07-24 03:49:10 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: rainbow trout, sperm, shotgun proteomics, proteome |
Contact List
Joanna Nynca |
contact affiliation | Department of Gamete and Embryo Biology |
contact email | j.nynca@pan.olsztyn.pl |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/11/PXD000355 |
Repository Record List
[ + ]
[ - ]
- PRIDE
- 31940
- InstrumentRef: INSTRUMENT_1
- Label: slice 1
- ModificationList: monohydroxylated residue, neutral loss, iodoacetamide derivatized residue,
- Name: Biological replicate 4
- PublicationRef: pending
- 31939
- InstrumentRef: INSTRUMENT_1
- Label: slice 1
- ModificationList: iodoacetamide derivatized residue, monohydroxylated residue, neutral loss,
- Name: Biological replicate 3
- PublicationRef: pending
- 31938
- InstrumentRef: INSTRUMENT_1
- Label: slice 1
- ModificationList: iodoacetamide derivatized residue, monohydroxylated residue, neutral loss,
- Name: Biological replicate 2
- PublicationRef: pending
- 31934
- InstrumentRef: INSTRUMENT_1
- Label: slice 1
- ModificationList: iodoacetamide derivatized residue, monohydroxylated residue, neutral loss,
- Name: Biological replicate 2
- PublicationRef: pending
- 31935
- InstrumentRef: INSTRUMENT_1
- Label: slice 12
- ModificationList: iodoacetamide derivatized residue, monohydroxylated residue, neutral loss,
- Name: Biological replicate 3
- PublicationRef: pending
- 31933
- InstrumentRef: INSTRUMENT_1
- Label: slice 1
- ModificationList: iodoacetamide derivatized residue, monohydroxylated residue, neutral loss,
- Name: Biological replicate 1
- PublicationRef: pending
- 31936
- InstrumentRef: INSTRUMENT_1
- Label: slice 1
- ModificationList: iodoacetamide derivatized residue, monohydroxylated residue, neutral loss,
- Name: Biological replicate 4
- PublicationRef: pending
- 31937
- InstrumentRef: INSTRUMENT_1
- Label: slice 1
- ModificationList: monohydroxylated residue, iodoacetamide derivatized residue, neutral loss,
- Name: Biological replicate 1
- PublicationRef: pending