PXD000355

PXD000355 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of rainbow trout sperm proteins
Description	Proteomics represents a powerful tool for the analysis of fish spermatozoa, since these cells are transcriptionally inactive. The aim of the present study was to generate an inventory of the most prominent rainbow trout sperm proteins with the use of one-dimensional electrophoresis prefractionation combined with performance liquid chromatography electrospray ionization tandem mass spectrometry. This study provides the first in-depth analysis of the rainbow trout sperm proteome, with a total of 204 identified proteins. We found that rainbow trout spermatozoa are equipped with functionally diverse proteins related to energetic metabolism, signal transduction, protein turnover, transport, cytoskeleton, oxidative injures and stress and reproduction. The availability of a catalogue of rainbow trout sperm proteins provides a crucial tool for the understanding of fundamental molecular processes in fish spermatozoa for ongoing research in the development of novel markers of sperm quality and for the optimization of short- and long-term sperm preservation procedures.
HostingRepository	PRIDE
AnnounceDate	2014-07-24
AnnouncementXML	Submission_2014-07-24_03:49:09.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000355
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joanna Nynca
SpeciesList	scientific name: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri); NCBI TaxID: 8022;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model: LTQ; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-22 04:08:39	ID requested
1	2013-11-05 01:35:39	announced
1	2013-11-06 07:15:43	announced
2	2014-04-02 06:56:11	announced	Updated publication reference for PubMed record(s): 24687995.
⏵ 3	2014-07-24 03:49:10	announced	Updated project metadata.

Nynca J, Arnold GJ, Fr, ö, hlich T, Otte K, Ciereszko A, Proteomic identification of rainbow trout sperm proteins. Proteomics, 14(12):1569-73(2014) [pubmed]

curator keyword: Biological

submitter keyword: rainbow trout, sperm, shotgun proteomics, proteome

Joanna Nynca
contact affiliation	Department of Gamete and Embryo Biology
contact email	j.nynca@pan.olsztyn.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/11/PXD000355

PRIDE project URI

• PRIDE
  1. PXD000355
     1. Label: PRIDE project
     2. Name: Proteomic identification of rainbow trout sperm proteins