PXD000275 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteome of MCF7 breast cancer cells treated with estradiol and BI2536 |
Description | Triple SILAC phosphoproteome experiment of hormone deprived MCF7 cells treated with estradiol (E2) in presence or absence of the PLK1 inhibitor BI2536. Conditions were as follows: light (L) population: 1h DMSO, 30min EtOH; medium (M) population: 1h DMSO, 30min E2; heavy (H) population: 1h BI2536, 30min E2. Biological replicates: A and B. Phosphopeptides were enriched from soluble and insoluble fraction using a two step enrichment protocol consiting of strong cation exchange and TiO2 affinity chromatography. The peptides were analyzed on an Orbitrap Velos mass spectrometer linked to an online nanoflow HPLC system via a nanoelectrospray ion source and fragmented via higher-energy C-trap dissociation (HCD). Analysis was carried out using MaxQuant (v. 1.2.2.5) with standard settings including STY phosphporylation as variable modification. |
HostingRepository | PRIDE |
AnnounceDate | 2016-06-25 |
AnnouncementXML | Submission_2016-06-25_00:30:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Wierer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue; 6x(13)C labeled residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2013-06-05 01:31:32 | ID requested | |
1 | 2016-06-24 02:09:17 | announced | |
⏵ 2 | 2016-06-25 00:30:27 | announced | Updated project metadata. |
Publication List
Wierer M, Verde G, Pisano P, Molina H, Font-Mateu J, Di Croce L, Beato M, PLK1 signaling in breast cancer cells cooperates with estrogen receptor-dependent gene transcription. Cell Rep, 3(6):2021-32(2013) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: MCF7, BI2536, PLK1, phosphorylation, SILAC, Estradiol, gene regulation, phosphoproteomics |
Contact List
Miguel Beato |
contact affiliation | Gene Regulation Stem Cells and Cancer Program, Center for Genomic Regulation (CRG), Dr. Aiguader 88, 08003 Barcelona, Spain Institució Catalana de Recerca i Estudis Avançats (ICREA), Pg. Lluis Companys 23, 08010 Barcelona, Spain |
contact email | miguel.beato@crg.eu |
lab head | |
Michael Wierer |
contact affiliation | Gene Regulation Program |
contact email | mwierer@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000275
- Label: PRIDE project
- Name: Phosphoproteome of MCF7 breast cancer cells treated with estradiol and BI2536