PXD000275

PXD000275 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome of MCF7 breast cancer cells treated with estradiol and BI2536
Description	Triple SILAC phosphoproteome experiment of hormone deprived MCF7 cells treated with estradiol (E2) in presence or absence of the PLK1 inhibitor BI2536. Conditions were as follows: light (L) population: 1h DMSO, 30min EtOH; medium (M) population: 1h DMSO, 30min E2; heavy (H) population: 1h BI2536, 30min E2. Biological replicates: A and B. Phosphopeptides were enriched from soluble and insoluble fraction using a two step enrichment protocol consiting of strong cation exchange and TiO2 affinity chromatography. The peptides were analyzed on an Orbitrap Velos mass spectrometer linked to an online nanoflow HPLC system via a nanoelectrospray ion source and fragmented via higher-energy C-trap dissociation (HCD). Analysis was carried out using MaxQuant (v. 1.2.2.5) with standard settings including STY phosphporylation as variable modification.
HostingRepository	PRIDE
AnnounceDate	2016-06-25
AnnouncementXML	Submission_2016-06-25_00:30:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Wierer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue; 6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-06-05 01:31:32	ID requested
1	2016-06-24 02:09:17	announced
⏵ 2	2016-06-25 00:30:27	announced	Updated project metadata.

Wierer M, Verde G, Pisano P, Molina H, Font-Mateu J, Di Croce L, Beato M, PLK1 signaling in breast cancer cells cooperates with estrogen receptor-dependent gene transcription. Cell Rep, 3(6):2021-32(2013) [pubmed]

curator keyword: Biomedical

submitter keyword: MCF7, BI2536, PLK1, phosphorylation, SILAC, Estradiol, gene regulation, phosphoproteomics

Miguel Beato
contact affiliation	Gene Regulation Stem Cells and Cancer Program, Center for Genomic Regulation (CRG), Dr. Aiguader 88, 08003 Barcelona, Spain Institució Catalana de Recerca i Estudis Avançats (ICREA), Pg. Lluis Companys 23, 08010 Barcelona, Spain
contact email	miguel.beato@crg.eu
lab head
Michael Wierer
contact affiliation	Gene Regulation Program
contact email	mwierer@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD000275

PRIDE project URI

• PRIDE
  1. PXD000275
     1. Label: PRIDE project
     2. Name: Phosphoproteome of MCF7 breast cancer cells treated with estradiol and BI2536