PXD000262-1

PXD000262 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Characterisation of PRR11
Description	Protein degradation provides an important regulatory mechanism used to control cell cycle progression and many other cellular pathways. To comprehensively analyze the spatial control of protein degradation in U2OS osteosarcoma cells, we have combined drug treatment and SILAC-based quantitative mass spectrometry with subcellular and protein fractionation. The resulting data set analyzed more than 74,000 peptides, corresponding to ~5000 proteins, from nuclear, cytosolic, membrane, and cytoskeletal compartments. These data identified rapidly degraded proteasome targets, such as PRR11 and highlighted a feedback mechanism resulting in translation inhibition, induced by blocking the proteasome. We show this is mediated by activation of the unfolded protein response. We observed compartment-specific differences in protein degradation, including proteins that would not have been characterized as rapidly degraded through analysis of whole cell lysates. Bioinformatic analysis of the entire data set is presented in the Encyclopedia of Proteome Dynamics, a web-based resource, with proteins annotated for stability and subcellular distribution.
HostingRepository	PRIDE
AnnounceDate	2013-07-24
AnnouncementXML	Submission_2013-07-24_07:21:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vackar Afzal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; deamidated residue; monohydroxylated lysine
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2013-05-22 03:03:48	ID requested
⏵ 1	2013-07-23 08:43:21	announced
⏵ 1	2014-07-25 01:12:38	announced
2	2013-07-24 07:21:23	announced	Add reference

Publication List

Larance M, Ahmad Y, Kirkwood KJ, Ly T, Lamond AI, Global subcellular characterization of protein degradation using quantitative proteomics. Mol Cell Proteomics, 12(3):638-50(2013) [pubmed]

Larance M, Ahmad Y, Kirkwood KJ, Ly T, Lamond AI, Global subcellular characterization of protein degradation using quantitative proteomics. Mol Cell Proteomics, 12(3):638-50(2013) [pubmed]

Keyword List

submitter keyword: U2OS, Cyclohexamide, MG132

submitter keyword: U2OS, Cyclohexamide, MG132

Contact List

Vackar Afzal
contact affiliation	School of Life Sciences
contact email	v.z.afzal@dundee.ac.uk

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/07/PXD000262

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/07/PXD000262