PXD000190-1

PXD000190 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics of miR-148a in gastric cancer cells
Description	A quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins in AGS cancer cells. For proteomic analysis, cells were treated with miR-148a mimic (Pre-miR-148a) or miR-148a negative control (miR-CTL) and the downstream protein expression level (Pre-miR-148a/miR-CTL) were quantified using iTRAQ approach. Bioinformatics pipeline: The peak list in the resultant MS/MS spectra were generated by Mascot Distiller v2.1.1.0  and searched using Mascot v2.2 against the International Protein Index (IPI) human database (v. 3.64, 84032 sequences). The Mascot search parameters were +-0.1 Da for MS tolerance, +-0.1 Da for MS/MS mass tolerance, allowances for two missed cleavages, and variable modifications of deamidation (NQ), oxidation (M), iTRAQ (N terminal), iTRAQ (K), and MMTS (C). Protein quantitation were calculated using the Multi-Q software v1.6.5.4 with a dynamic range filter of ion count > 30.
HostingRepository	PRIDE
AnnounceDate	2013-07-24
AnnouncementXML	Submission_2013-07-24_07:14:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000190
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chia-Wei Hu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; deamidated residue; monohydroxylated residue; neutral loss: 63.998285; methylthiolated residue
Instrument	Synapt G2-S MS; Synapt G2 HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2013-03-22 03:15:31	ID requested
⏵ 1	2013-07-24 07:14:34	announced
⏵ 1	2014-07-28 00:32:25	announced
2	2014-08-08 02:34:12	announced	Updated publication reference for PubMed record(s): 23869555.

Dataset with its publication pending

submitter keyword: quantitative proteome, iTRAQ, miR-148a

Chien-Wei Tseng
contact attribute	angus8921@gmail.com
contact affiliation	National Taiwan University
contact email	angus8921@gmail.com
Hsueh-Fen Juan
contact attribute	yukijuan@gmail.com
contact affiliation	National Taiwan University
contact email	yukijuan@gmail.com
Chia-Wei Hu
contact affiliation	Institute of molecular and cellular biology
contact email	hkmq8195@gmail.com

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• PRIDE
  1. 28857
     1. InstrumentRef: INSTRUMENT_1
     2. Label: iTRAQ analysis of miR-148a bio rep 2
     3. ModificationList: iTRAQ4plex-116 reporter+balance reagent acylated residue, deamidated residue, monohydroxylated residue, neutral loss, methylthiolated residue,
     4. Name: iTRAQ analysis of miR-148a
     5. PublicationRef: pending
  2. 28858
     1. InstrumentRef: INSTRUMENT_2
     2. Label: iTRAQ of miR-148a
     3. ModificationList: iTRAQ4plex-116 reporter+balance reagent acylated residue, monohydroxylated residue, neutral loss, deamidated residue, methylthiolated residue,
     4. Name: iTRAQ analysis of miR-148a-treated gatric cancer cells
     5. PublicationRef: pending