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PXD000190

PXD000190 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative Proteomics of miR-148a in gastric cancer cells
DescriptionA quantitative proteomics combined with stable isotope labeling was applied to identify the global profile of miR-148a-regulated downstream proteins in AGS cancer cells. For proteomic analysis, cells were treated with miR-148a mimic (Pre-miR-148a) or miR-148a negative control (miR-CTL) and the downstream protein expression level (Pre-miR-148a/miR-CTL) were quantified using iTRAQ approach. Bioinformatics pipeline: The peak list in the resultant MS/MS spectra were generated by Mascot Distiller v2.1.1.0  and searched using Mascot v2.2 against the International Protein Index (IPI) human database (v. 3.64, 84032 sequences). The Mascot search parameters were +-0.1 Da for MS tolerance, +-0.1 Da for MS/MS mass tolerance, allowances for two missed cleavages, and variable modifications of deamidation (NQ), oxidation (M), iTRAQ (N terminal), iTRAQ (K), and MMTS (C). Protein quantitation were calculated using the Multi-Q software v1.6.5.4 with a dynamic range filter of ion count > 30.
HostingRepositoryPRIDE
AnnounceDate2014-07-28
AnnouncementXMLSubmission_2014-08-08_02:34:11.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD000190
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChia-Wei Hu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdeamidated residue; monohydroxylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
Instrumentinstrument model: synapt G2 HDMS; MALDI Synapt G2 HDMS; instrument model: Q-TOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02013-03-22 03:15:31ID requested
12013-07-24 07:14:34announced
12014-07-28 00:32:25announced
22014-08-08 02:34:12announcedUpdated publication reference for PubMed record(s): 23869555.
Publication List
Hu CW, Tseng CW, Chien CW, Huang HC, Ku WC, Lee SJ, Chen YJ, Juan HF, Quantitative proteomics reveals diverse roles of miR-148a from gastric cancer progression to neurological development. J Proteome Res, 12(9):3993-4004(2013) [pubmed]
Keyword List
submitter keyword: quantitative proteome, iTRAQ, miR-148a
Contact List
Chia-Wei Hu
contact affiliationInstitute of molecular and cellular biology
contact emailhkmq8195@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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