PXD000163-1
PXD000163 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Anti-FLAG-affinity purification of Umbrea associated factors |
Description | Anti-FLAG-affinity purification of Umbrea associated factors from nuclear extracts of Drosophila Schneider cells expressing FLAG-HA-Umbrea. Anti-FLAG -affinity purifications from Schneider cell nuclear extracts not expressing any FLAG-tagged protein served as a control SDS-PAGE separting both samples, 9 slices per sample, reduction and alkylation, digestion with trypsin. LC-MS on a Ultimate3000-LTQ Orbitrap XL Raw data were analysed using the MaxQuant 1.2.2.5 software package. Identified proteins were considered as interaction partners if their MaxQuant intensities displayed a greater than 10 fold enrichment compared to control anti-FLAG purifications from L2-4 nuclear extracts not expressing any FLAG-tagged protein. |
HostingRepository | PRIDE |
AnnounceDate | 2013-10-03 |
AnnouncementXML | Submission_2013-10-03_00:23:07.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andreas Thomae |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | iodoacetamide derivatized residue; monohydroxylated residue; acetylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2013-02-27 03:12:32 | ID requested | |
⏵ 1 | 2013-10-03 00:23:13 | announced | |
⏵ 1 | 2013-10-03 00:25:22 | announced | |
2 | 2014-07-25 00:55:43 | announced | Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Umbrea, Fruitfly, Drosophila melanogaster |
Contact List
Andreas Thomae | |
---|---|
contact affiliation | Molecular Biology |
contact email | andreas.thomae@med.uni-muenchen.de |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/10/PXD000163 |