PXD000163

PXD000163 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Anti-FLAG-affinity purification of Umbrea associated factors
Description	Anti-FLAG-affinity purification of Umbrea associated factors from nuclear extracts of Drosophila Schneider cells expressing FLAG-HA-Umbrea. Anti-FLAG -affinity purifications from Schneider cell nuclear extracts not expressing any FLAG-tagged protein served as a control SDS-PAGE separting both samples, 9 slices per sample, reduction and alkylation, digestion with trypsin. LC-MS on a Ultimate3000-LTQ Orbitrap XL Raw data were analysed using the MaxQuant 1.2.2.5 software package. Identified proteins were considered as interaction partners if their MaxQuant intensities displayed a greater than 10 fold enrichment compared to control anti-FLAG purifications from L2-4 nuclear extracts not expressing any FLAG-tagged protein
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:18:50.533.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Thomae
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2013-02-27 03:12:32	ID requested
1	2013-10-03 00:23:13	announced
1	2013-10-03 00:25:22	announced
2	2014-07-25 00:55:43	announced	Updated project metadata.
⏵ 3	2024-10-22 04:18:51	announced	2024-10-22: Updated project metadata.

Publication List

10.1126/science.1234393;
Ross BD, Rosin L, Thomae AW, Hiatt MA, Vermaak D, de la Cruz AF, Imhof A, Mellone BG, Malik HS, Stepwise evolution of essential centromere function in a Drosophila neogene. Science, 340(6137):1211-4(2013) [pubmed]

10.1126/science.1234393;

Ross BD, Rosin L, Thomae AW, Hiatt MA, Vermaak D, de la Cruz AF, Imhof A, Mellone BG, Malik HS, Stepwise evolution of essential centromere function in a Drosophila neogene. Science, 340(6137):1211-4(2013) [pubmed]

Keyword List

submitter keyword: Fruitfly,Umbrea, Drosophila melanogaster

submitter keyword: Fruitfly,Umbrea, Drosophila melanogaster

Contact List

Andreas Thomae
contact affiliation	Molecular Biology
contact email	andreas.thomae@med.uni-muenchen.de
lab head
Andreas Thomae
contact affiliation	Molecular Biology
contact email	andreas.thomae@med.uni-muenchen.de
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/10/PXD000163
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/10/PXD000163

PRIDE project URI

Repository Record List

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