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PXD000096

PXD000096 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRIPchipSRM C. elegans miR58 targets (Jovanovic, et al.)
DescriptionTo discover new miRNA targets, we generated a C. elegans transgenic line expressing a functional, N-terminally tandem affinity purification (TAP)-tagged ALG-1 protein (TAP::ALG-1 strain) (see Methods for details).We next purified TAP::ALG-1 complexes from mixed-stage TAP::ALG-1 transgenic and wild-type animals (serving as a mock control) and hybridized the associated mRNAs to twocolor microarrays.
HostingRepositoryPeptideAtlas
AnnounceDate2014-03-11
AnnouncementXMLSubmission_2014-03-12_13:28:14.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLukas Reiter
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListisotope labeled residue
Instrument4000 QTRAP
Dataset History
RevisionDatetimeStatusChangeLog Entry
02012-12-11 16:27:52ID requested
12012-12-11 16:28:23announced
22014-03-12 13:28:15announcedupdate LabHead
Publication List
Jovanovic M, Reiter L, Clark A, Weiss M, Picotti P, Rehrauer H, Frei A, Neukomm LJ, Kaufman E, Wollscheid B, Simard MJ, Miska EA, Aebersold R, Gerber AP, Hengartner MO, RIP-chip-SRM--a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans. Genome Res, 22(7):1360-71(2012) [pubmed]
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted
Contact List
Lukas Reiter
contact affiliationUZH
contact emailboardsofcanada@gmx.ch
dataset submitter
Michael O. Hengartner
contact affiliationUZH
contact emailmichael.hengartner@imls.uzh.ch
lab head
Full Dataset Link List
PeptideAtlas dataset URI
PASSEL experiment URI
PASSEL transition group browser URI