PXD076035

PXD076035 is an original dataset announced via ProteomeXchange.

Title	Determination of the peroxisome proteome of pathogenic stage Histoplasma capsulatum via Proximity Labeling
Description	During infection, Histoplasma capsulatum exists primarily as an intracellular pathogen of phagocytes where it overcomes host defense mechanisms and replicates in the phagosome. This intracellular proliferation of Histoplasma yeasts requires the function of the fungal peroxisome for multiple, incompletely defined roles, with early pathogenesis functions dependent on the proper trafficking of peroxisome matrix proteins carrying a type 1 peroxisome-targeting signal (PTS1). To better understand the potential roles of peroxisomes during Histoplasma infection, we identified the constituents of the peroxisome proteome of pathogenic phase Histoplasma yeasts through proximity labeling using a peroxisome-targeted TurboID biotin ligase. Comparative proteomics using peroxisome- or cytosol-localized TurboID identified 90 high confidence peroxisome proteins. To validate the peroxisome proteome, a subset of nine putative peroxisome proteins were localized to the peroxisome using fluorescent protein fusions. To empirically define which matrix proteins of the peroxisome proteome depend on the PTS1 or PTS2 import pathways, peroxisome proteomes were also defined for Histoplasma yeasts lacking the Pex5 or Pex7 cytosolic PTS1 or PTS2 receptors. This assigned over half of the proteins to either the PTS1- or PTS2-dependent peroxisome import pathways and refined the Histoplasma PTS1 tripeptide consensus sequence. The peroxisomal proteome of Histoplasma yeast during macrophage infection was ultimately determined to provide relevance to Histoplasma’s primary pathogenic niche. The identities of the proteins comprising the peroxisome proteome include several enzymes for canonical peroxisome-localized biochemical pathways (e.g., fatty acid utilization and siderophore biosynthesis) but also represent several potential novel functions for the organelle. The defined peroxisome proteome of pathogenic-phase Histoplasma yeasts, particularly the set of proteins imported via the PTS1 import pathway, thus establishes the important foundation for understanding how peroxisomes promote intracellular pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2026-06-04
AnnouncementXML	Submission_2026-06-04_10:53:53.922.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD076035
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Peter Brechting
SpeciesList	scientific name: Histoplasma ohiense; NCBI TaxID: NEWT:2902605;
ModificationList	ubiquitinylated lysine; phosphorylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-23 12:51:33	ID requested
⏵ 1	2026-06-04 10:53:54	announced

10.6019/PXD076035;

10.1128/MBIO.00771-26;

submitter keyword: Histoplasma, Proximity Labeling, Fungi, Peroxisomes

Chad Alan Rappleye
contact affiliation	Rappleye Lab, Department of Microbiology, Ohio State University, United States
contact email	rappleye.1@osu.edu
lab head
Peter Brechting
contact affiliation	The Ohio State University
contact email	peter.brechting@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD076035
     1. Label: PRIDE project
     2. Name: Determination of the peroxisome proteome of pathogenic stage Histoplasma capsulatum via Proximity Labeling