The demo dataset of ApuQuant for testing consists of DIA data acquired by Orbitrap Astral mass spectrometer. This submission includes two demo datasets together with the corresponding DIA-NN identification results. Demo Data I contains raw files from low-input samples with four technical replicates, as well as the corresponding DIA-NN identification results. Demo Data II contains raw files from dead cell samples with three technical replicates and large-sized cell samples with three technical replicates, together with the corresponding DIA-NN identification results. These datasets are provided as representative test data for evaluating the performance of ApuQuant on challenging DIA proteomics samples, including low-input samples and biologically distinct cell populations. The raw mass spectrometry files and search results can be used for demonstration, benchmarking, and reproducibility assessment of the ApuQuant workflow.