PXD075425

PXD075425 is an original dataset announced via ProteomeXchange.

Title	VAMP8 function reveals tight linkage between endocytic recycling and endocytosis
Description	Clathrin-mediated endocytosis (CME) is a multistage process that involves the initiation and stabilization of clathrin-coated pits (CCPs) that invaginate and finally detach from the plasma membrane to form clathrin-coated vesicles (CCVs). Given that SNARE proteins are essential for downstream vesicle targeting and fusion events, their recruitment into nascent CCVs has been suggested to be a prerequisite for CME progression. However, which and how SNARE proteins regulate CME remains to be explored. Here, we showed that siRNA-mediated knockdown of the R-SNARE, VAMP8 impairs CCP initiation, stabilization and invagination and strongly inhibits CME. Mechanistically, recruitment of VAMP8 to CCVs is not required for CME. Instead,depletion of VAMP8 inhibits recycling of endocytic cargoes and as exemplified here by transferrin receptor, skews their trafficking toward lysosomal degradation. VAMP8 depletion therefore indirectly impairs CCV formation and inhibits CME by depleting endocytic cargo. Overall, our study provides new insights into the crosstalk between endocytosis and endocytic recycling of CME cargo and demonstrates the critical role for cargo recruitment in stabilizing nascent CCPs to regulate CME.
HostingRepository	iProX
AnnounceDate	2026-03-09
AnnouncementXML	Submission_2026-03-09_19:06:02.317.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yaohui He
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-09 19:04:19	ID requested
⏵ 1	2026-03-09 19:06:02	announced

Dataset with its publication pending

submitter keyword: clathrin-mediated endocytosis, endocytic recycling, VAMP8, transferrin, lysosome

Yaohui He
contact affiliation	University of South China
contact email	2024001050@usc.edu.cn
lab head
Yaohui He
contact affiliation	University of South China
contact email	969220692@qq.com
dataset submitter

iProX dataset URI