PXD074286

PXD074286 is an original dataset announced via ProteomeXchange.

Title	Jiayi Huatu Decoction mitigates intestinal fibrosis via a gut microbiota–metabolite axis
Description	Ulcerative colitis (UC), a chronic inflammatory bowel condition, lacks effective treatments. We aimed to examine the therapeutic potential of Jiayi Huatu Decoction (JHD), a traditional Chinese formula, in treating UC in mice, focusing on its modulation of gut microbiota and metabolites. JHD significantly alleviated intestinal fibrosis and chronic inflammation. Mechanistically, JHD promoted beneficial gut bacteria, specifically Faecalibaculum rodentium and Akkermansia muciniphila. Integrated metabolomics and proteomics identified excessive 2-piperidone and taurocholic acid accumulation as colonic fibrosis and inflammation drivers. F. rodentium-derived butyric acid suppressed copper amine oxidase 3, reducing 2-piperidone production, while A. muciniphila-derived 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine (LysoPE16:0) activated farnesoid X receptor (FXR) to decrease taurocholic acid. Genetic ablation of FXR abolished LysoPE16:0 anti-fibrotic and anti-inflammatory activities. JHD exerts therapeutic effects by modulating gut microbiota and supporting F. rodentium and A. muciniphila propagation, which synergistically regulate extracellular matrix deposition and cell adhesion signaling pathways through specific metabolites, offering a strategy for UC treatment.
HostingRepository	iProX
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-09_19:56:36.713.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chun Peng
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-09 19:56:09	ID requested
⏵ 1	2026-02-09 19:56:37	announced

Dataset with its publication pending

submitter keyword: Gut microbiota–metabolite axis, Traditional Chinese medicine, Ulcerative colitis, Intestinal fibrosis

Dongmei Chen
contact affiliation	National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agricultural University
contact email	chendongmei@mail.hzau.edu.cn
lab head
Chun Peng
contact affiliation	National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agricultural University
contact email	pengchun1998@163.com
dataset submitter

iProX dataset URI