PXD074285

PXD074285 is an original dataset announced via ProteomeXchange.

Title	Characterization of Vibrio outer membrane vesicle-induced immune responses in Litopenaeus vannamei based on comparative proteomics analysis
Description	Outer membrane vesicles (OMVs) derived from Vibrio species serve as important delivery vehicles for effector molecules and play critical roles in host–pathogen interactions. However, the systemic immune response patterns elicited by Vibrio-derived OMVs in the Pacific white shrimp (Litopenaeus vannamei) remain poorly characterized. In this study, we investigated the immune response features of L. vannamei following stimulation with OMVs derived from two Vibrio parahaemolyticus strains responsible for acute hepatopancreas necrosis disease (AHPND) and translucent postlarvae disease (TPD), respectively. In vivo imaging and hemocyte uptake assays revealed that OMVs preferentially accumulated in the hepatopancreas and intestine, two major immune-related tissues, accompanied by pronounced hemocyte-mediated active internalization. Following in vivo injection, shrimp exhibited marked immune activation, as evidenced by alterations in total hemocyte count, redistribution of hemocyte subpopulations, and elevated reactive oxygen species (ROS) levels, indicating a systemically activated immune state. At the transcriptional level, the hepatopancreas and intestine displayed distinct tissue-specific immune response patterns to the two OMVs, which were accompanied by coordinated changes in antioxidant enzyme activities. Histopathological examination further demonstrated that both VpAHPND OMVs and VpTPD OMVs induced varying degrees of structural damage in the hepatopancreas and intestinal tissues. Comparative proteomic analysis of the hepatopancreas provided additional insights into the potential molecular mechanisms underlying shrimp-OMVs interactions. OMVs stimulation triggered coordinated intracellular xenobiotic clearance pathways involving cytoskeleton remodeling-mediated endocytosis-lysosome trafficking, autophagic degradation, and ubiquitin-dependent proteasomal processes, progressed toward programmed cell fate determination characterized by necroptosis, and ultimately drove systemic defensive responses associated with immune recognition and redox homeostasis. Collectively, this study systematically delineates the tissue targeting, cellular responses, and molecular signatures of L. vannamei following stimulation with Vibrio-derived OMVs. These findings suggest that Vibrio OMVs act as critical immunomodulatory signals capable of inducing coordinated systemic and tissue-specific immune responses, providing a foundation for future investigations into their roles in Vibrio-associated diseases and immune regulation in crustaceans.
HostingRepository	iProX
AnnounceDate	2026-02-10
AnnouncementXML	Submission_2026-02-09_19:55:14.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lihan Wang
SpeciesList	scientific name: Penaeus vannamei; NCBI TaxID: 6689;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-09 19:54:58	ID requested
⏵ 1	2026-02-09 19:55:15	announced

Dataset with its publication pending

submitter keyword: Outer membrane vesicles, Litopenaeus vannamei, Innate immunity, Immune response, Proteomics

Mengqiang Wang
contact affiliation	Hainan Key Laboratory of Tropical Aquatic Germplasm (Hainan Seed Industry Laboratory), Sanya Oceanographic Institution, Ocean University of China
contact email	wangmengqiang@ouc.edu.cn
lab head
Lihan Wang
contact affiliation	Hainan Key Laboratory of Tropical Aquatic Germplasm (Hainan Seed Industry Laboratory), Sanya Oceanographic Institution, Ocean University of China
contact email	2648181506@qq.com
dataset submitter

iProX dataset URI