PXD073786 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Label-free quantitative proteomics of extracellular vesicles from BT549 cells following 3-day reversine treatment Label-free quantitative proteomics of extracellular vesicles from BT549 cells following 3-day reversine treatment |
| Description | Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles Triple-negative breast cancer (TNBC) is characterised by high rates of chromosomal instability (CIN) and rewired intercellular communication driven by both soluble factors and extracellular vesicles (EVs). To assess how CIN might affect EV-mediated signalling in TNBC, we studied the EV landscape of TNBC cell lines with induced CIN. We find that CIN leads to increased secretion of EVs and that these EVs promote cell migration of recipient cells. EVs are enriched for extracellular matrix (ECM) proteins, including EFEMP1. Indeed, modulation of EFEMP1 levels in EVs significantly alters migration behaviour of EV-treated cells. We show that EFEMP1 expression is regulated by STAT1, and that EVs from STAT1-deficient cells no longer promote migration, which can be rescued by overexpression of EFEMP1 in STAT1-null cells. Xenografting TNBC cells with EFEMP1 enriched cells promotes migration in zebrafish embryos, suggesting that EFEMP1 expression is a factor that promotes metastasis. Together, our results identify a CIN-associated EV program in TNBC and highlight EFEMP1 as a potential therapeutic target to impair EV-driven tumour cell migration. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-10 |
| AnnouncementXML | Submission_2026-03-10_09:03:56.551.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Justina Clarinda Wolters |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-29 08:53:19 | ID requested | |
| ⏵ 1 | 2026-03-10 09:03:57 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: BT549 |
| triple-negative breast cancer |
| reversine |
| chromosomal instability |
| extracellular vesicles |
| exosomes |
| label-free quantification |
| LC-MS/MS |
| Q Exactive Plus |
Contact List
| JC Wolters |
|---|
| contact affiliation | Department of Pediatrics, University Medical Center Groningen, University of Groningen, The Netherlands |
| contact email | j.c.wolters@ucmg.nl |
| lab head | |
| Justina Clarinda Wolters |
|---|
| contact affiliation | UMCG |
| contact email | j.c.wolters@umcg.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073786
- Label: PRIDE project
- Name: Label-free quantitative proteomics of extracellular vesicles from BT549 cells following 3-day reversine treatment Label-free quantitative proteomics of extracellular vesicles from BT549 cells following 3-day reversine treatment
