PXD073650

PXD073650 is an original dataset announced via ProteomeXchange.

Title	Macrophage SH3GLB1 is required for Rab7-1 mediated mitophagy in inflammation during acute lung injury
Description	Acute lung injury (ALI) accompanied by an inflammatory response is an important complication after drowning. Macrophage activation and polarization are implicated in the inflammatory process of lipopolysaccharide (LPS)-induced ALI (LPS-ALI), but little is known about drowning-induced ALI (drowning-ALI). SH3GLB1 is a member of the endophilin family and has been shown to be involved in mitochondrial morphological changes and autophagy. However, its role in ALI remains unclear. Here,we performed single-cell profiling of lung immune cells isolated from the lungs of drowning-ALI mouse models. We found that the regulation of macrophages in drowning-ALI was similar to that in LPS-ALI. Specifically, SH3GLB1 was highly expressed in macrophages of drowning-ALI mouse models and was related to inflammation. Furthermore, SH3GLB1 deletion ameliorated LPS-ALI or drowning-ALI. In contrast, the restoration of SH3GLB1 expression provoked LPS-ALI and drowning-ALI. Mechanistically, SH3GLB1 was shown to interact with Rab7 to contribute to mitophagy, which resulted in mitochondrial dysfunction. Overall, these findings indicated that SH3GLB1 is required for Rab7-mediated mitophagy in inflammation during ALI and could be a novel target for lung protection.
HostingRepository	PRIDE
AnnounceDate	2026-02-10
AnnouncementXML	Submission_2026-02-09_16:46:48.166.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fa guang Jin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-27 10:03:22	ID requested
⏵ 1	2026-02-09 16:46:48	announced

Dataset with its publication pending

submitter keyword: Acute lung injury

Drowning

SH3GLB1

Rab7

Mitophagy

Fa guang
contact affiliation	Department of Pulmonary and Critical Care Medicine, Tangdu Hospital, Fourth Military Medical University, No. 569, Xinsi Road, Baqiao District, Xi'an City, Shaanxi Province
contact email	jinfag@fmmu.edu.cn
lab head
Fa guang Jin
contact affiliation	Fourth Military Medical University
contact email	jinfag@fmmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073650
     1. Label: PRIDE project
     2. Name: Macrophage SH3GLB1 is required for Rab7-1 mediated mitophagy in inflammation during acute lung injury