PXD073205

PXD073205 is an original dataset announced via ProteomeXchange.

Title	Opsonisation improves duel proteomic analysis of Burkholderia cenocepacia infections. -DIA infection +/- Opsonisation of T6SS and TecA
Description	The opportunistic Cystic Fibrosis (CF) pathogen Burkholderia cenocepacia is associated with severe lung infections. Within the CF lung, macrophages are both a crucial reservoir and a key mediator of the intense inflammatory response characteristic of B. cenocepacia infections. Despite the importance of macrophages for B. cenocepacia pathogenesis, there is a paucity of insight into how B. cenocepacia internalisation and replication impacts both the host and B. cenocepacia proteomes. A key limitation for understanding the proteomic changes during intracellular replication of B. cenocepacia has been the low infectivity of this pathogen, resulting in the generation of mixed cell populations dominated by uninfected cells within in vitro models. Using antibody-mediated opsonization, we show that by improving the efficiency and uniformity of B. cenocepacia internalization this enhances dual proteomic analysis. Comparing opsonized and non-opsonized infections, we show that changes in both host and internalized B. cenocepacia can be assessed in a single experimental framework. At the proteome level, opsonization dramatically improves the detection of protein changes, enhancing the detection of pro-inflammatory signaling and macrophage activation markers. Utilizing this dual proteomic approach, we assess the impact of the B. cenocepacia type 6 secretion system (T6SS) and the T6SS effector TecA at 3 and 24 hours post infection demonstrating that the presence/activities of the T6SS or TecA do not greatly modulate the proinflammatory response of THP-1 cells. Thus, this work demonstrates a simple means for enhancing proteomic analysis of B. cenocepacia infections enabling dual proteomic studies.
HostingRepository	PRIDE
AnnounceDate	2026-05-13
AnnouncementXML	Submission_2026-05-13_12:46:23.061.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nichollas Scott
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-18 09:25:54	ID requested
⏵ 1	2026-05-13 12:46:23	announced

Dataset with its publication pending

submitter keyword: Macrophages,Burkholderia cenocepacia, Data-independent acquisition, Opsonization, Dual-proteomics, Burkholderia, Proteomics

Nichollas E. Scott
contact affiliation	Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne 3000, Australia
contact email	nichollas.scott@unimelb.edu.au
lab head
Nichollas Scott
contact affiliation	University of Melbourne
contact email	nichollas.scott@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073205

PRIDE project URI

• PRIDE
  1. PXD073205
     1. Label: PRIDE project
     2. Name: Opsonisation improves duel proteomic analysis of Burkholderia cenocepacia infections. -DIA infection +/- Opsonisation of T6SS and TecA