PXD072439

PXD072439 is an original dataset announced via ProteomeXchange.

Title	Lactate-Mediated Cross-Talk between KRT15 and GPR68 drives Type 2 Immunity in ECRSwNP
Description	Eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP) is a refractory inflammatory disease driven by type 2 immunity, yet the mechanistic link between epithelial stress and immune activation remains elusive. Here, we identify a pathogenic expansion of KRT5⁺KRT15⁺ basal epithelial cells that undergo a metabolic switch to aerobic glycolysis, resulting in lactate overproduction and microenvironment acidification. This acidic niche activates the proton-sensing receptor GPR68 on infiltrating CD4⁺ T cells, triggering a Gq–IP1–IKK/ERK signaling cascade that promotes Th2 differentiation and cytokine release. Genetic ablation of GPR68 in CD4⁺ T cells or its pharmacological inhibition with a natural antagonist, 3-Bn-6-Bnid-DKP, ameliorated type 2 inflammation and pathology in vivo. Thus, we define a lactate-GPR68 axis that metabolically couples epithelial dysfunction to adaptive type 2 immunity in ECRSwNP
HostingRepository	iProX
AnnounceDate	2025-12-26
AnnouncementXML	Submission_2025-12-26_01:12:13.489.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongjie Du
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-26 01:11:51	ID requested
⏵ 1	2025-12-26 01:12:14	announced

Dataset with its publication pending

submitter keyword: Lactate, ECRSwNP, GPR68, immunity

Ming Xia
contact affiliation	Shandong Provincial Hospital Affiliated to Shandong First Medical University
contact email	LCcheng211314@126.com
lab head
Hongjie Du
contact affiliation	Shandong Provincial Hospital Affiliated to Shandong First Medical University
contact email	duhongjie0316@126.com
dataset submitter

iProX dataset URI