PXD072384

PXD072384 is an original dataset announced via ProteomeXchange.

Title	The large-scale interactions between bile acids and proteins
Description	Characterization of small-molecule and protein interactions is essential for elucidating protein biochemical activity, regulatory mechanisms, and the intricate relationships within biological signaling pathways. A variety of methodologies have been developed to identify interactions between proteins and small molecules. Small-molecule probe-based pull-down assays represent a well-established and intuitive approach for identifying proteins that interact with specific small molecules. However, large-scale probe synthesis remains challenging due to its complex procedures and labor-intensive nature, thereby hindering the comprehensive mapping of small-molecule-protein interaction networks. In this study, we introduce a strategy employing solid-phase assisted probe synthesis and 96-well based target capture. This approach capitalizes on the immobilization of small molecules on a solid support, enabling efficient and high-throughput synthesis of diverse probes. Applying this method, we synthesized 13 chemically cleavable bile acid probes and identified a total of 7,306 interaction pairs between 13 bile acids and 1883 proteins. This large dataset provides a wealth of information about the interactions of bile acids with cellular proteins. Furthermore, we conducted a comparative analysis between SD (standard diet) and HFM (high-fat diet-induced obesity model) groups. This analysis led to the identification of 2,639 differential bile acid-protein pairs, highlighting the changes in these interactions in response to dietary fat intake. These results demonstrate the potential of our method to reveal novel insights into the complex interplay between small molecules and proteins in different biological contexts.
HostingRepository	iProX
AnnounceDate	2025-12-24
AnnouncementXML	Submission_2025-12-23_19:19:51.364.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinlong Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	oxidation
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-23 19:19:30	ID requested
⏵ 1	2025-12-23 19:19:51	announced

Dataset with its publication pending

submitter keyword: Proteomics，Small molecule-protein interactions，Mass spectrometry, Probe synthesis, Bile acids

Jinlong Wang
contact affiliation	Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences
contact email	jinlongwang@ucas.ac.cn
lab head
Jinlong Wang
contact affiliation	Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences
contact email	jinlongwang@ucas.ac.cn
dataset submitter

iProX dataset URI