PXD072378

PXD072378 is an original dataset announced via ProteomeXchange.

Title	TFAM-Associated Mitochondrial Dynamics and Metabolic Reprogramming Regulate Microglial Polarization: Temporal and Causal Perspectives
Description	The polarization state of microglia exerts an influence on neuroinflammation and neural tissue repair after injury. Modulating microglial polarization is emerging as a potential therapeutic strategy for various types of neural injuries and neurodegenerative diseases. However, the causal relationship between microglial polarization and mitochondrial dynamics, which include mitochondrial fusion and fission, remains to be fully clarified. Our study demonstrates that mitochondrial fusion promoter M1 promotes mitochondrial fusion in mouse microglial cells, leading to reduced glycolysis and increased fatty acid oxidation, and this metabolic reprogramming impacts microglial polarization. Additionally, in both cellular and animal experiments, it was observed that knocking down mitochondrial transcription factor A (TFAM) results in increased mitochondrial fission, decreased fatty acid β-oxidation, enhanced glycolysis, and promotes the polarization of microglia towards the pro - inflammatory M1 phenotype. In conclusion, our study has, for the first time, provided evidence that TFAM may play a role in the regulation of mitochondrial dynamics. Furthermore, we provide a detailed elucidation of the chronological sequence and underlying causal relationships among mitochondrial dynamics, mitochondrial metabolic reprogramming, and microglial polarization. These findings offer novel targets and strategies for the treatment of various neural injuries and neurodegenerative diseases.
HostingRepository	iProX
AnnounceDate	2025-12-24
AnnouncementXML	Submission_2025-12-23_18:41:49.184.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuxuan Zhang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-23 18:41:24	ID requested
⏵ 1	2025-12-23 18:41:49	announced

Dataset with its publication pending

submitter keyword: TFAM, Mitochondria, Metabolism, Fatty Acids, Glycolysis, Microglia, Cell Polarization.

Jianyuan Jiang
contact affiliation	Department of Orthopedics, Huashan Hospital, Fudan University
contact email	jiangyuanjiang01@126.com
lab head
Yuxuan Zhang
contact affiliation	Department of Orthopedics, Huashan Hospital, Fudan University
contact email	zhangyuxuan92@163.com
dataset submitter

iProX dataset URI