PXD072358

PXD072358 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	N6-adenosine methylation enhances nuclear mRNA export through METTL3 and NUP93
Description	Nuclear export of messenger RNAs (mRNAs) through nuclear pore complexes (NPCs) is a critical step in gene expression. Although N6-adenosine methylation (m6A) has been implicated in this process, the underlying mechanism remains obscure. Here, using single-molecule imaging, we demonstrate that m6A markedly accelerates nuclear export of messenger ribonucleoproteins (mRNPs) by increasing export efficiency and shortening export time through NPCs. We further show that the m6A methyltransferase METTL3 localizes at NPCs and functionally associates with the nucleoporin NUP93 to promote efficient export of m6A-modified mRNPs. Disruption of this functional association between METTL3 and NUP93 substantially impairs overall mRNP export efficiency. Notably, a steroid-resistant nephrotic syndrome (SRNS)-associated NUP93 variant (c.1162C>T, p.Arg388Trp) fails to associate with METTL3, resulting in defective nuclear export of key methylated mRNAs required for kidney function. Together, our findings define an m6A-METTL3-NUP93 regulatory axis for nuclear mRNA export with broad implications for human disease.
HostingRepository	MassIVE
AnnounceDate	2025-12-23
AnnouncementXML	Submission_2025-12-23_11:25:40.863.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susan T. Weintraub
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-23 11:16:31	ID requested
⏵ 1	2025-12-23 11:25:41	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: N6-adenosine methylation, METTL3, NUP93, nuclear mRNA export, data-independent acquisition MS, DatasetType:Proteomics

submitter keyword: N6-adenosine methylation, METTL3, NUP93, nuclear mRNA export, data-independent acquisition MS, DatasetType:Proteomics

Contact List

Jihoon Lee
contact affiliation	University of Texas Health Science Center San Antonio
contact email	leej23@uthscsa.edu
lab head
Susan T. Weintraub
contact affiliation	UT Health Science Center San Antonio
contact email	weintraub@uthscsa.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100322/