PXD071376

PXD071376 is an original dataset announced via ProteomeXchange.

Title	Hemostatic and platelet proteins associate with bleeding grade and time-to-major bleed in hemato-oncological patients
Description	Bleeding crises are common complications in hemato-oncological patients undergoing chemotherapy and stem cell transplantation. Platelet transfusions are frequently administered to treat and prevent bleeding events at low platelet counts. However, the association between thrombocytopenia and bleeding events remains unclear, arguing for an improved understanding of pathophysiological changes underpinning bleeding events. Therefore, this study aims to identify plasma protein levels associated with bleeding events in a hemato-oncological patient population using unbiased mass spectrometry-based plasma profiling. Plasma samples and clinical records from 176 hemato-oncological patients included in the Pathogen Reduction Evaluation & Predictive Analytical Rating Score (PREPAReS) study were analyzed. Out of 600 identified proteins, abundance levels of 55 proteins were found to be associated with bleeding severity at time of sampling. Among these, a large cluster of platelet proteins (n = 48) as well as coagulation factor 7 (F7) and vitamin-K dependent protein C (PROC) were observed. Interestingly, nine out of these 55 proteins were also associated with time-to-major bleeding event (WHO grade 2 or higher). Platelet protein levels correlated with platelet counts, but not with white cell counts nor platelet transfusions. Taken together, our results suggest that lower abundance of platelet- and coagulation proteins in plasma are associated with increased bleeding grade and time-to-major bleeding events in hemato-oncological patients. This highlights the potential for improved hemostatic monitoring of hemato-oncological patients at risk of bleeding, beyond platelet count alone.
HostingRepository	PRIDE
AnnounceDate	2026-04-02
AnnouncementXML	Submission_2026-04-01_17:57:37.289.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eva Smit
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-28 10:37:00	ID requested
⏵ 1	2026-04-01 17:57:37	announced

10.1016/J.BVTH.2026.100144;

submitter keyword: bleeding, hemostasis,Plasma proteomics, hemato-oncological

Maartje van den Biggelaar
contact affiliation	Department of Molecular Hematology, Sanquin Research, Amsterdam.
contact email	m.vandenbiggelaar@sanquin.nl
lab head
Eva Smit
contact affiliation	Sanquin
contact email	e.smit@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD071376

PRIDE project URI

• PRIDE
  1. PXD071376
     1. Label: PRIDE project
     2. Name: Hemostatic and platelet proteins associate with bleeding grade and time-to-major bleed in hemato-oncological patients