PXD069579

PXD069579 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiling of an ALS Drosophila Model Reveals the Impact of Scaffold Degrader P3 on Head Tissue
Description	This project presents a quantitative proteomic analysis of Drosophila melanogaster head tissue expressing human FUS-P525L, using data-independent acquisition (DIA) mass spectrometry. To enable identification of the human transgene, mass spectrometry data were analyzed against a custom database containing the Drosophila melanogaster proteome (UniProt UP000000803) and the human FUS sequence. Head samples were processed through protein extraction, acetone precipitation, and in-solution tryptic digestion. LC-MS/MS data were acquired on a Thermo Scientific instrument and analyzed with the DIA-NN software (v1.8.1), with FDR controlled at 1%. The dataset enables the investigation of protein expression changes, including the transgenic human FUS, under P3 treatment.
HostingRepository	iProX
AnnounceDate	2025-10-17
AnnouncementXML	Submission_2025-10-17_00:34:27.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YingChen Zhou
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-17 00:34:14	ID requested
⏵ 1	2025-10-17 00:34:27	announced

Dataset with its publication pending

submitter keyword: Stress Granule, Drosophila melanogaster, FUS

JiaHeng Tan
contact affiliation	Sun Yat-sen University
contact email	tanjiah@mail.sysu.edu.cn
lab head
YingChen Zhou
contact affiliation	Sun Yat-sen University
contact email	zhouych35@mail2.sysu.edu.cn
dataset submitter

iProX dataset URI