PXD069335 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Low-resolution FAIMS for increased peptide coverage in low-load and single-cell proteomics |
| Description | Field Asymmetric Ion Mobility Spectrometry (FAIMS) is an indispensable tool in single-cell and low-input proteomics. Here, we show that tuning FAIMS resolution via electrode temperature modulation improves sensitivity. We demonstrate that lowering FAIMS resolution broadens the compensation voltage window, thereby increasing ion transmission. This significantly improves ion counts, quantitative precision and peptide identifications by 25 - 34%. These results were mirrored when analyzing single cell samples. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-01 |
| AnnouncementXML | Submission_2026-04-01_05:49:36.650.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Manuel Matzinger |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-10 14:57:07 | ID requested | |
| ⏵ 1 | 2026-04-01 05:49:37 | announced | |
Publication List
Keyword List
| submitter keyword: low-input proteomics, ion mobility filter, single cell proteomics,FAIMS |
Contact List
| Karl Mechtler |
|---|
| contact affiliation | Proteomics Technology Hub, IMP, Vienna Biocenter |
| contact email | karl.mechtler@imp.ac.at |
| lab head | |
| Manuel Matzinger |
|---|
| contact affiliation | IMP / University of Vienna |
| contact email | manuel.matzinger@imp.ac.at |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD069335 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069335
- Label: PRIDE project
- Name: Low-resolution FAIMS for increased peptide coverage in low-load and single-cell proteomics
