PXD069333

PXD069333 is an original dataset announced via ProteomeXchange.

Title	Zinc stimulation of phytoplankton in a low carbon dioxide, coastal Antarctic environment: evidence for the Zn hypothesis
Description	The ocean acts as a carbon sink, absorbing carbon from the atmosphere and resulting in substantial uptake of anthropogenic CO2 emissions. As biological processes in the oceans such as net primary production (NPP) contribute significantly to this sink, understanding how they will shift in response to increasing atmospheric CO2 is necessary to project future ocean carbon storage capacity. Macronutrient and micronutrient resource limitation within the oceans regulates NPP, and while some micronutrients such as zinc (Zn) are present at very low concentrations, their ability to limit NPP has remained unclear. Zn is a key micronutrient used by phytoplankton for a multitude of metabolic functions, yet there have been few observations of its influence on natural oceanic phytoplankton populations. In this study, we observed Zn limitation of growth in the natural phytoplankton community of Terra Nova Bay, Antarctica, in addition to primary iron (Fe) limitation. Shipboard incubation experiments amended with Zn and Fe resulted in significantly higher chlorophyll a content and dissolved inorganic carbon drawdown compared to Fe addition alone. Zn and Fe stress response proteins detected in incubation and environmental biomass provided independent verification of algal co-stress for these micronutrients. We consider total biomass and low surface ocean pCO2 as potential drivers of environmental Zn stress. This study definitively establishes that Zn limitation can occur in the modern oceans, opening up new possibility space in our understanding of nutrient regulation of NPP through geologic time, and we consider the future of oceanic Zn limitation in the face of climate change.
HostingRepository	PRIDE
AnnounceDate	2025-10-17
AnnouncementXML	Submission_2025-10-17_08:06:21.083.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069333
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew McIlvin
SpeciesList	scientific name: marine metagenome; NCBI TaxID: NEWT:408172;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-10 13:23:01	ID requested
⏵ 1	2025-10-17 08:06:21	announced

10.6019/PXD069333;

submitter keyword: metaproteomics, ocean environment, zinc

Mak Saito
contact affiliation	Woods Hole Oceanographic Institute Woods Hole, MA 02543
contact email	msaito@whoi.edu
lab head
Matthew McIlvin
contact affiliation	Woods Hole Oceanographic Inst.
contact email	mmcilvin@whoi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069333
     1. Label: PRIDE project
     2. Name: Zinc stimulation of phytoplankton in a low carbon dioxide, coastal Antarctic environment: evidence for the Zn hypothesis