PXD069261

PXD069261 is an original dataset announced via ProteomeXchange.

Title	Development of a chemoproteomic platform to identify the sites of (homo)citrullination within complex proteomes
Description	Citrullination and homocitrullination of arginine and lysine can significantly impact protein structure and function. Citrullination of arginine is an enzymatic modification catalyzed by the Protein Arginine Deiminases (PADs). Homocitrullination of lysine is a non-enzymatic modification that occurs in the presence of high concentrations of cyanate. Both post-translational modifications are elevated in Rheumatoid Arthritis (RA) and other inflammatory diseases. Moreover, autoantibodies targeting these PTMs are associated with the development of RA. Identifying arginine and lysine residues that are hypersensitive to these modifications is critical for deepening our understanding of the functional effects of (homo)citrullination. Current methods use phenylglyoxal-biotin for the protein-level identification of (homo)citrullinated proteins, however, this platform does not inform on the exact site of citrullination. Herein we describe the development of a desthiobiotin-phenylglyoxal (DB-PG) probe, which can be used to selectively enrich and subsequently release (homo)citrullinated peptides for the site-specific identification of (homo)citrullinated arginines and lysines. Coupled with TMT quantification, this method monitors citrullination in ~800 arginine residues and ranks ~1400 lysine residues by homocitrullination sensitivity. Projecting forward, this platform will enable the comprehensive analysis of (homo)citrullination in complex proteomes.
HostingRepository	PRIDE
AnnounceDate	2026-03-10
AnnouncementXML	Submission_2026-03-09_20:05:25.252.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Mann
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	N6-carbamoyl-L-lysine; L-citrulline
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-09 12:43:43	ID requested
⏵ 1	2026-03-09 20:05:25	announced

10.1021/ACSCHEMBIO.5C00694;

submitter keyword: phenylglyoxal, chemoproteomics, desthiobiotin,Citrulline, homocitrulline

Eranthie Weerapana
contact affiliation	Boston College
contact email	eranthie@bc.edu
lab head
Sarah Mann
contact affiliation	Boston College
contact email	mannsc@bc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD069261

PRIDE project URI

• PRIDE
  1. PXD069261
     1. Label: PRIDE project
     2. Name: Development of a chemoproteomic platform to identify the sites of (homo)citrullination within complex proteomes