PXD068569

PXD068569 is an original dataset announced via ProteomeXchange.

Title	Nucleoside Binding by a Surface Lipoprotein Governs Conjugative ICE Acquisition in Ruminant Mycoplasmas
Description	This project aims to investigate whether the surface lipoproteins CDS14 and P48, both required for ICEA-mediated conjugation in Mycoplasma agalactiae, physically interact during mating. CDS14 is encoded by ICEA and constitutively expressed in donor cells, whereas P48 is expressed by both donor and recipient strains as part of an ABC transporter system. Using reciprocal co-immunoprecipitation (co-IP) assays combined with mass spectrometry, we will test the hypothesis that CDS14 and P48 form a functional complex that facilitates ICEA transfer.
HostingRepository	PRIDE
AnnounceDate	2025-12-19
AnnouncementXML	Submission_2025-12-19_01:23:51.809.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre STELLA
SpeciesList	scientific name: Mycoplasma agalactiae; NCBI TaxID: NEWT:2110;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-19 06:01:33	ID requested
⏵ 1	2025-12-19 01:23:52	announced

Dataset with its publication pending

submitter keyword: Mycoplasma

Integrative and conugative elements (ICE)

Lipoprotein P48

Odile Schiltz
contact affiliation	Proteomics and Mass Spectrometry of Biomolecules, ProteoToul Research Proteomics Facility of Toulouse, ProteoToul Services Proteomics French Infrastructure, ProFI IPBS, CNRS, UMR 5089 205 route de Narbonne BP 64182 31077 Toulouse Cedex France
contact email	Odile.Schiltz@ipbs.fr
lab head
Alexandre STELLA
contact affiliation	CNRS
contact email	alexandre.stella@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD068569

PRIDE project URI

• PRIDE
  1. PXD068569
     1. Label: PRIDE project
     2. Name: Nucleoside Binding by a Surface Lipoprotein Governs Conjugative ICE Acquisition in Ruminant Mycoplasmas