⮝ Full datasets listing
PXD068396
PXD068396 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PrPC-facilitated cell-signaling activates phospholipase Cgamma1 and triggers an Arc/Arg3.1 response in mouse neurons and human iPSC-derived neurons |
| Description | Synapse loss is an early feature of prion disease, yet the underlying drivers are poorly understood. We recently revealed evidence of neuronal hyperactivity in prion-infected animal models. Herein we identified increased Arc/Arg3.1 in patients with prion disease, suggesting heightened neuronal activity also occurs in human prion-affected brain. To determine the signaling events initiated by prion aggregates (PrPSc), we developed a disease model in which human iPSC-derived excitatory neurons (iNs) are stimulated with a PrPSc-mimetic antibody that binds the cellular prion protein. Within two hours, we detected an Arc response together with transcriptomic changes previously reported in prion-infected mice. Proteomics, RNA sequencing, and a phosphokinase array revealed altered phosphorylation changes of PLC-gamma1, ERK1/2, and EGFR as PrPC-triggered cell signaling events accompanying the Arc/Arg3.1 response. These results suggest that PrPC ligands, including PrPSc, may trigger rapid signaling events linked to neuronal hyperactivity in human neurons, and indicate PLC-gamma1 as a potential interventional target. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-04-01 |
| AnnouncementXML | Submission_2026-04-01_16:42:12.849.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Daniel McClatchy |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-15 17:06:30 | ID requested | |
| ⏵ 1 | 2026-04-01 16:42:13 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Prion Disease, Neurodegeneration, Proteomics, Alzheimer's disease, protein misfolding, DatasetType:Proteomics |
Contact List
| Christina Sigurdson | |
|---|---|
| contact affiliation | UC San Diego |
| contact email | csigurdson@health.ucsd.edu |
| lab head | |
| Daniel McClatchy | |
| contact affiliation | TSRI |
| contact email | dmcclat@scripps.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099196/ |
