PXD068076

PXD068076 is an original dataset announced via ProteomeXchange.

Title	Induced pluripotent stem cell-derived macrophages as a model for human inflammasome signalling
Description	Macrophage models are a mainstay of inflammasome research, however current human in vitro macrophage models have significant limitations. Here we generate induced pluripotent stem cell (iPSC)-derived macrophages (iMacs) to study inflammasome signalling and benchmark them with human monocyte-derived macrophages (HMDMs). We confirm that iMacs express high levels of macrophage markers and are highly phagocytic. Whole cell proteomics analysis shows that iMacs express many inflammasome sensors and related proteins, and in functional assays iMacs respond to multiple inflammasome stimuli. The NLRP3 inflammasome is strongly activated in iMacs and we find that nigericin alone activates NLRP3. The non-canonical inflammasome does not require a priming step in iMacs as caspase-4 is constitutively expressed. High levels of NAIP/NLRC4 inflammasome activation are also observed in response to needle toxin. Finally, unlike HMDM, iMacs activate NLRP1. Therefore, we demonstrate that iMacs are a physiologically relevant and highly tractable model to study human inflammasome signalling and regulation.
HostingRepository	PRIDE
AnnounceDate	2026-06-04
AnnouncementXML	Submission_2026-06-04_08:41:57.330.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohammad Arefian
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-04 09:13:12	ID requested
⏵ 1	2026-06-04 08:41:57	announced

Dataset with its publication pending

submitter keyword: Protein aggregation capture (PAC), Innate immunity, Lipopolysaccharide (LPS), Mass spectrometry,Macrophages, Proteomics, Inflammasomes

Ben C. Collins
contact affiliation	School of Biological Sciences, Queen’s University of Belfast, Belfast, UK
contact email	Ben.Collins@qub.ac.uk
lab head
Mohammad Arefian
contact affiliation	Researcher
contact email	m.arefian@qub.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD068076

PRIDE project URI

• PRIDE
  1. PXD068076
     1. Label: PRIDE project
     2. Name: Induced pluripotent stem cell-derived macrophages as a model for human inflammasome signalling