PXD065844

PXD065844 is an original dataset announced via ProteomeXchange.

Title	Unlocking the Zn-enriching Potential of Industrial Yeast Strains – an experimental journey from metal analysis to proteomics
Description	Nutritional supplements like trace element enriched yeasts become increasingly popular to overcome the worldwide problem of zinc (Zn) deficiency. Unlike Selenium-enriched yeast, Zn-enriched yeasts (ZnY) have not yet been authorised in the European Union, as their evaluation is still ongoing demanding more comprehensive data regarding the Zn species present in ZnY. This study aimed to screen ten different industrial yeast strains regarding their Zn-enrichment quota with further characterisation of selected strains using spectroscopic and proteomic approaches. Microfermentation experiments on 10 industrial yeasts showed Zn levels spanning 0.06 – 51 pg/cell. Large-scale fermentation in bioreactors was carried out with two strains excelling in either biomass or Zn accumulation. A combination of inductively coupled mass spectrometry (ICP-MS) and various spectroscopic methods confirmed the Zn-enrichment, while suggesting that fractions of the Zn accumulated on the cell surface with simultaneously high values of phosphorus being present. Speciation via X-ray absorption spectroscopy (XANES) analyses also revealed, that Zn is coordinated in close proximity to P-O-ligands and to amino acid ligands in both strains. Proteomic analyses showed that ZnY cells moved from a Zap1-governed zinc balance to an intracellular excess response, implying cellular zinc uptake. This study demonstrates that in a Zn-excess medium there is variability between different industrial yeast strains in terms of their Zn-accumulation capacity, cellular Zn-localisation and regulatory response by expression of Zn-binding proteins. The presented findings contribute to optimizing industrial fermentation processes for producing Zn-rich yeast biomass and enhance the understanding of Zn regulation in yeast, aiding in the approval of Zn-enriched yeasts for supplements and novel food applications.
HostingRepository	PRIDE
AnnounceDate	2025-12-28
AnnouncementXML	Submission_2025-12-28_02:03:46.792.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Muenzner
SpeciesList	scientific name: Saccharomyces pastorianus; NCBI TaxID: NEWT:27292; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-07 05:39:07	ID requested
⏵ 1	2025-12-28 02:03:47	announced

Dataset with its publication pending

submitter keyword: zinc speciation, XANES,Zinc supplement, zinc-enriched yeast

Markus Ralser
contact affiliation	Charité Universitätsmedizin Berlin
contact email	markus.ralser@charite.de
lab head
Julia Muenzner
contact affiliation	Charite University Medicine, Berlin, Germany
contact email	julia.muenzner@charite.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065844
     1. Label: PRIDE project
     2. Name: Unlocking the Zn-enriching Potential of Industrial Yeast Strains – an experimental journey from metal analysis to proteomics