PXD065732

PXD065732 is an original dataset announced via ProteomeXchange.

Title	Human whole blood and plasma from healthy participants comparing VAMS optimised sample preparation methods to other conventional methods.
Description	Whole blood and plasma samples from healthy volunteers were processed using our novel VAMS-optimised technique involving selective washing steps to deplete high-abundance proteins. Samples were analysed using shotgun liquid chromatography mass spectrometry with data-independent acquisition (DIA) mode to comprehensively screen the blood proteome. The VAMS-optimised method dramatically enhanced protein detection, identifying 3024 proteins from plasma samples (4-fold increase over liquid samples) and over 3496 proteins from whole blood samples (2.1-fold increase). Analysis revealed 281 differentially expressed proteins between liquid and VAMS-optimised plasma samples, with 64% upregulated in the VAMS-optimised samples, including previously suppressed low-abundance proteins such as platelet and cytoskeletal proteins. Similarly, whole blood analysis identified 928 differentially expressed proteins between conventional and VAMS-optimised methods. Our rapid and reproducible VAMS-optimised blood preparation method enabled the production of high-quality proteomic data with mean coefficients of variation below 11% from small volumes (30 µL) of complex samples that typically require extensive depletion or fractionation prior to proteomic analysis. The method proved superior to direct VAMS processing and performed comparably to commercial protein corona bead assays while offering greater flexibility across sample types.
HostingRepository	PRIDE
AnnounceDate	2025-12-24
AnnouncementXML	Submission_2025-12-24_04:36:57.811.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elisabeth Karsten
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-03 04:20:36	ID requested
⏵ 1	2025-12-24 04:36:58	announced

10.1021/ACS.JPROTEOME.5C00687;

submitter keyword: Human, plasma, VAMS, anticoagulants, whole blood, storage, protein corona beads

Ben Herbert
contact affiliation	Chief Scientific Officer at Sangui Bio
contact email	Ben@sanguibio.com
lab head
Elisabeth Karsten
contact affiliation	Sangui Bio
contact email	elisabeth@sanguibio.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065732
     1. Label: PRIDE project
     2. Name: Human whole blood and plasma from healthy participants comparing VAMS optimised sample preparation methods to other conventional methods.