PXD064101
PXD064101 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Signaling Pathway Alterations in Hearts of a Porcine Model Harboring a B-Myosin Heavy Chain (MYH7-R403Q) Gene Variant |
| Description | The disease-causing myosin variant (MYH7-403Q) is linked to hypertrophic cardiomyopathy (HCM). We carried out a research study of signaling pathways in heart samples from control wild-type (WT) GE Yucatán mini-pigs and their littermates harboring the gene variant, MYH7-R403Q. Our approach permits the determination of adverse signaling pathways involved in different regions of a translationally relevant heart without the effects of intervention. We examined the left ventricular free wall (LV), endocardium (EN), and coronary arteries (CA) from 5 transgenic and 5 wild-type mini-pig littermates to determine alterations in global phosphorylation and protein abundance. Digested peptides from 6-7 months old mixed-sex mini-pigs were isobarically labeled; 95% were phospho-enriched, and 5% were used as the unmodified (total) fraction. The phospho-enriched and unmodified fractions were injected into an Orbitrap Fusion Lumos and analyzed using PEAKS Studio and Ingenuity Pathway Analysis. Surprisingly, we found no significant changes in the phospho-peptide and unmodified protein abundances in CA. Compared to WT, both LV and EN samples displayed minor changes in phosphorylation and significant changes in unmodified proteins. Bioinformatic analysis revealed that pathways associated with mechano-signaling between cardiomyocytes and the extracellular matrix and inflammation were altered in LV and EN samples. In addition, EN samples had larger differences in pathways related to metabolic dysfunction compared to LV. Our findings provide a translational understanding of signaling pathways altered in the MYH7-R403Q gene variant. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-10-17 |
| AnnouncementXML | Submission_2025-10-17_04:51:49.178.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Chad M. Warren |
| SpeciesList | scientific name: Sus scrofa domesticus; common name: domestic pig; NCBI TaxID: 9825; |
| ModificationList | Carbamidomethyl; L-methionine sulfoxide; Deamidated; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-05-20 04:25:19 | ID requested | |
| ⏵ 1 | 2025-10-17 04:51:49 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Proteomics, Hypertrophic Cardiomyopathy, Fibrosis, Mechano-Signaling, Inflammation, Metabolic Dysfunction, DatasetType:Proteomics |
Contact List
| R. John Solaro | |
|---|---|
| contact affiliation | University of Illinois-Chicago |
| contact email | solarorj@uic.edu |
| lab head | |
| Chad M. Warren | |
| contact affiliation | University of Illinois-Chicago |
| contact email | cmwarren@uic.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097929/ |
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