PXD062929
PXD062929 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | High-throughput chemical proteomics workflow for profiling protein citrullination dynamics |
| Description | Citrullination is a post-translational modification implicated in autoimmune and inflammatory diseases but remains challenging to analyze biochemically. Its low stoichiometry and the lack of enrichment tools have hindered its global and site-specific profiling. Here, we developed a high-throughput chemical proteomics workflow for citrullination profiling with enhanced sensitivity and specificity. This workflow employs glyoxal-based derivatization of citrullinated peptides, followed by a click reaction with a biotin conjugate, featuring a cleavable linker that enhances peptide recovery and following identification. We examined the derivatization efficiency using synthetic citrullinated peptides and demonstrated its sensitivity and reproducibility in complex biological samples. Mouse brain digest samples were then used for direct comparison to existent enrichment tools. Dose-dependent ionomycin treatments of isolated neutrophils from fresh blood allowed us to profile citrullination during neutrophil extracellular trap (NET) formation. Known and novel sites on different histone proteins, as well as structural proteins mediating nuclear and cytoskeletal reorganization, were found to be regulated. These findings illuminate citrullination dynamics in NET formation and establish a platform for exploring its roles in diverse systems. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-23 |
| AnnouncementXML | Submission_2025-12-23_07:01:15.090.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rebecca Meelker Gonzalez |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | L-citrulline; unknown modification |
| Instrument | Orbitrap Fusion Lumos; Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-04-14 05:15:49 | ID requested | |
| ⏵ 1 | 2025-12-23 07:01:15 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Citrullination, Enrichment, Chemical derivatization, Neutrophil activation, Mouse brain, Histone, DatasetType:Proteomics |
Contact List
| Dr. Chien-Yun Lee | |
|---|---|
| contact affiliation | Young Investigator Group: Mass Spectrometry in Systems Neurosciences, School of Life Sciences,Technical University of Munich |
| contact email | chienyun.lee@tum.de |
| lab head | |
| Rebecca Meelker Gonzalez | |
| contact affiliation | LeeLab, YIG, School of Life Sciences, Technische Universitaet Muenchen (TUM) |
| contact email | r.meelker@tum.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097617/ |
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