PXD061392

PXD061392 is an original dataset announced via ProteomeXchange.

Title	Proteomic insights into age-related changes in laser capture microdissected glomeruli of human kidney
Description	Kidney aging is characterized by loss of glomeruli, predominately in the superficial cortex, with a resultant decline in glomerular filtration rate and an increased risk of various kidney-related diseases. The early molecular alterations in glomeruli with the aging process is not well studied. In this study, we combined laser capture microdissection and mass spectrometry-based unbiased proteomic analysis of non-sclerosed, non-ischemic glomeruli in the superficial cortex from young and old individuals who underwent a radical nephrectomy for a tumor to understand the age-related molecular changes in glomeruli. As expected, kidneys from older individuals had lower eGFR, less total kidney cortex, more glomerulosclerosis, and more arteriosclerosis. Quantitative proteomic analysis showed several proteins involved in the extracellular matrix reorganization as over expressed in old compared to young individuals. Functional characterization of the differential expressed proteins showed that overexpressed proteins were enriched in mitochondrial translation elongation and termination whereas under expressed proteins were enriched in RNA splicing, mRNA transport and protein processing. Overexpression of TIMP3, APOA4, SNCG, NT5E and GPC6 were further confirmed by proteomic analysis on an independent cohort. After adjusting for age, expression of these proteins did not associate with low eGFR or chronic changes on histology or CT imaging of the kidneys. Overall, this study identified proteins that are associated with the normal aging process in otherwise normal appearing glomeruli.
HostingRepository	PRIDE
AnnounceDate	2025-12-23
AnnouncementXML	Submission_2025-12-23_05:25:24.799.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-03 05:29:01	ID requested
⏵ 1	2025-12-23 05:25:26	announced

10.34067/kid.0000000986;

Mangalaparthi KK, Sachdeva GS, Shaik AA, Venkataraman S, Mullan AF, Pujari GP, Madden BJ, Asghar MS, Sharma V, Alexander MP, Larson NB, Denic A, Pandey A, Rule AD, Spatial Mass Spectrometry-Based Proteomic Analysis of Normal-Appearing Glomeruli from Young and Old Adults. Kidney360, 6(12):2071-2081(2025) [pubmed]

submitter keyword: NT5E, Renal disorders, Laser capture microdissection, TIMP3,Aging, Extracellular matrix, diaPASEF

Akhilesh Pandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN
contact email	pandey.akhilesh@mayo.edu
lab head
Akhilesh Pandey
contact affiliation	Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905
contact email	pandey.akhilesh@mayo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD061392

PRIDE project URI

• PRIDE
  1. PXD061392
     1. Label: PRIDE project
     2. Name: Proteomic insights into age-related changes in laser capture microdissected glomeruli of human kidney