PXD061137

PXD061137 is an original dataset announced via ProteomeXchange.

Title	Neo N-termiome of SPG7, AFG3L2, SPG7 AFG3L2 and WT HeLa cells
Description	To understand how OMA1 affects mitochondrial proteostasis and stress responses, we performed a proteomic survey of OMA1-deficient cells for proteolytic substrates. We demonstrate that OMA1 cleaves the mitochondrial chaperone DNAJC15 facilitating its degradation by the mitochondrial m-AAA protease. The loss of DNAJC15 alters protein import by TIM23 protein translocases in the IM and limits the accumulation of OXPHOS-related mitochondrial matrix and IM proteins. Non-imported mitochondrial preproteins accumulate at the endoplasmic reticulum (ER) and trigger an ATF6-related unfolded protein response. These results demonstrate that OMA1 allows to adapt mitochondrial protein biogenesis to stress and reveal an intricate network of cellular stress responses to proteostasis disturbances. In this dataset, we determined the Neo N termiome of SPG7, AFG3L2 and SPG7 AFG3L2 knock out HeLa cells. The project is related to other identfiers PXD061131, PXD061134.
HostingRepository	PRIDE
AnnounceDate	2025-12-19
AnnouncementXML	Submission_2025-12-19_03:06:23.599.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hendrik Nolte
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-24 07:58:21	ID requested
⏵ 1	2025-12-19 03:06:24	announced

Dataset with its publication pending

submitter keyword: Human, NeoN, SPG7, HeLa, AFG3L2

Thomas Langer
contact affiliation	Department of Mitochondrial Proteostasis Joseph-Stelzmann-Str. 9b 50931 Cologne
contact email	tlanger@age.mpg.de
lab head
Hendrik Nolte
contact affiliation	Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany
contact email	h.nolte@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD061137

PRIDE project URI

• PRIDE
  1. PXD061137
     1. Label: PRIDE project
     2. Name: Neo N-termiome of SPG7, AFG3L2, SPG7 AFG3L2 and WT HeLa cells